Indole is an inter-species biofilm signal mediated by SdiA.
Indole is an inter-species biofilm signal mediated by SdiA.
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DOI：
10.1186/1471-2180-7-42
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发表时间：
2007-05-18
期刊：
影响因子：
4.2
通讯作者：
Wood TK
中科院分区：
文献类型：
--
作者：
Lee J;Jayaraman A;Wood TK
作为稳定期信号，吲哚被大肠杆菌大量分泌到丰富培养基中，并且已经显示出控制几种基因（例如，astD、tnaB、gabT）、多药输出菌和E.然而，其对生物膜形成的影响尚未得到充分研究。通过一系列的全局转录组分析，共聚焦显微镜，等基因突变体，和双物种生物膜，我们在这里表明，吲哚是一个无毒的信号，控制E。大肠杆菌生物膜通过抑制运动性，诱导群体感应信号自诱导物-1（SdiA）的传感器，和影响耐酸性（例如，hdeABD，gadABCEX）。同基因突变体显示这些相关蛋白与生物膜形成直接相关（例如，sdiA突变使生物膜形成增加了50倍），并且SdiA介导的转录被证明受到吲哚的影响。由于吲哚添加导致的运动性降低导致生物膜结构从分散的塔变为平坦的菌落。此外，还有12倍以上的E。大肠杆菌细胞在双物种生物膜中生长的假单胞菌细胞的存在下，工程表达甲苯o-单加氧酶（TOM，它转换吲哚到一个不溶性的靛蓝）比在生物膜与假单胞菌，不表达TOM由于22倍减少细胞外吲哚。此外，吲哚刺激假单胞菌中的生物膜形成。吲哚效应由SdiA和高丝氨酸内酯群体感应介导的进一步证据是，N-丁酰基-、N-己酰基-和N-辛酰基-L-高丝氨酸内酯的加入抑制E.大肠杆菌生物膜形成的野生型菌株，但没有与sdiA突变体。吲哚是种间信号，降低E。大肠杆菌生物膜通过SdiA和增加假单胞菌的那些。吲哚可以被操纵来控制生物膜形成的细菌的加氧酶，不合成它在一个双物种的生物膜。此外，E.大肠杆菌改变其生物膜以响应它不能合成的信号（高丝氨酸内酯），而假单胞菌响应它们不能合成的信号（吲哚）。
As a stationary phase signal, indole is secreted in large quantities into rich medium by Escherichia coli and has been shown to control several genes (e.g., astD, tnaB, gabT), multi-drug exporters, and the pathogenicity island of E. coli; however, its impact on biofilm formation has not been well-studied. Through a series of global transcriptome analyses, confocal microscopy, isogenic mutants, and dual-species biofilms, we show here that indole is a non-toxic signal that controls E. coli biofilms by repressing motility, inducing the sensor of the quorum sensing signal autoinducer-1 (SdiA), and influencing acid resistance (e.g., hdeABD, gadABCEX). Isogenic mutants showed these associated proteins are directly related to biofilm formation (e.g., the sdiA mutation increased biofilm formation 50-fold), and SdiA-mediated transcription was shown to be influenced by indole. The reduction in motility due to indole addition results in the biofilm architecture changing from scattered towers to flat colonies. Additionally, there are 12-fold more E. coli cells in dual-species biofilms grown in the presence of Pseudomonas cells engineered to express toluene o-monooxygenase (TOM, which converts indole to an insoluble indigoid) than in biofilms with pseudomonads that do not express TOM due to a 22-fold reduction in extracellular indole. Also, indole stimulates biofilm formation in pseudomonads. Further evidence that the indole effects are mediated by SdiA and homoserine lactone quorum sensing is that the addition of N-butyryl-, N-hexanoyl-, and N-octanoyl-L-homoserine lactones repress E. coli biofilm formation in the wild-type strain but not with the sdiA mutant. Indole is an interspecies signal that decreases E. coli biofilms through SdiA and increases those of pseudomonads. Indole may be manipulated to control biofilm formation by oxygenases of bacteria that do not synthesize it in a dual-species biofilm. Furthermore, E. coli changes its biofilm in response to signals it cannot synthesize (homoserine lactones), and pseudomonads respond to signals they do not synthesize (indole).
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影响因子：
3.2
作者：
Barrios, AFG;Zuo, RJ;Wood, TK
通讯作者：
Wood, TK
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3.2
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3.6
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3.6
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