Structural, Evolutionary, and Functional Analysis of the Class III Peroxidase Gene Family in Chinese Pear (Pyrus bretschneideri).
Structural, Evolutionary, and Functional Analysis of the Class III Peroxidase Gene Family in Chinese Pear (Pyrus bretschneideri).
复制标题
DOI：
10.3389/fpls.2016.01874
复制
发表时间：
2016
影响因子：
5.6
通讯作者：
Cai Y
中科院分区：
文献类型：
--
作者：
Cao Y;Han Y;Meng D;Li D;Jin Q;Lin Y;Cai Y
过氧化物酶（Peroxidases，PRXs）广泛存在于各种生物体内，根据其结构和功能可分为不同的类型。其中，Class III Peroxidase是植物特有的多基因家族，参与植物生长素的代谢、细胞壁的延伸和增厚以及木质素的形成等多种生理过程。通过对梨基因组数据库的检索，从白梨中鉴定出94个非冗余PRXs（PbPRXs）。随后，进行了系统发育关系，基因结构，保守基序和微同线性的分析。这些PbPRXs在梨的17条染色体上分布不均匀。此外，在这些PbPRX中还发生了26次片段重复，而只有1次串联重复，这表明片段重复是PbPRX家族扩大的主要原因。通过Ka/Ks分析，在27个重复的PbPRXs中，有26个经历了纯化选择。基于MEME分析，在PbPRXs中鉴定了20个基序，其中11个在梨中富集。从梨果实EST中共鉴定出41个表达基因。qRT-PCR结果表明，在梨果实发育过程中，5个PbPRXs在C亚组中的表达趋势与木质素含量的变化趋势一致。因此，我们推测这5个PbPRXs是参与木质素合成途径的候选基因。这些结果为梨PRX基因的进一步研究提供了有用的信息。
Peroxidases (PRXs) are widely existed in various organisms and could be divided into different types according to their structures and functions. Specifically, the Class III Peroxidase, a plant-specific multi-gene family, involves in many physiological processes, such as the metabolism of auxin, the extension and thickening of cell wall, as well as the formation of lignin. By searching the pear genome database, 94 non-redundant PRXs from Pyrus bretschneideri (PbPRXs) were identified. Subsequently, analysis of phylogenetic relationships, gene structures, conserved motifs, and microsynteny was performed. These PbPRXs were unevenly distributed among 17 chromosomes of pear. In addition, 26 segmental duplication events but only one tandem duplication were occurred in these PbPRXs, implying segmental duplication was the main contributor to the expansion of the PbPRX family. By the Ka/Ks analysis, 26 out of 27 duplicated PbPRXs has experienced purifying selection. Twenty motifs were identified in PbPRXs based on the MEME analysis, 11 of which were enriched in pear. A total of 41 expressed genes were identified from ESTs of pear fruit. According to qRT-PCR, the expression trends of five PbPRXs in subgroup C were consistent with the change of lignin content during pear fruit development. So we inferred that the five PbPRXs were candidate genes involved in the lignin synthesis pathway. These results provided useful information for further researches of PRX genes in pear.
登录
查看更多内容
影响因子：
3.5
作者：
Cao Y;Han Y;Li D;Lin Y;Cai Y
通讯作者：
Cai Y
影响因子：
4.5
作者：
Bruex A;Kainkaryam RM;Wieckowski Y;Kang YH;Bernhardt C;Xia Y;Zheng X;Wang JY;Lee MM;Benfey P;Woolf PJ;Schiefelbein J
通讯作者：
Schiefelbein J
影响因子：
3.7
作者：
Muthamilarasan M;Khandelwal R;Yadav CB;Bonthala VS;Khan Y;Prasad M
通讯作者：
Prasad M
影响因子：
4.1
作者：
Ooka, H;Satoh, K;Kikuchi, S
通讯作者：
Kikuchi, S
影响因子：
14.9
作者：
Bailey TL;Johnson J;Grant CE;Noble WS
通讯作者：
Noble WS