The staphylococcal accessory regulator, SarA, is an RNA-binding protein that modulates the mRNA turnover properties of late-exponential and stationary phase Staphylococcus aureus cells.
The staphylococcal accessory regulator, SarA, is an RNA-binding protein that modulates the mRNA turnover properties of late-exponential and stationary phase Staphylococcus aureus cells.
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DOI：
10.3389/fcimb.2012.00026
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发表时间：
2012
影响因子：
5.7
通讯作者：
Dunman PM
中科院分区：
文献类型：
--
作者：
Morrison JM;Anderson KL;Beenken KE;Smeltzer MS;Dunman PM
mRNA周转的调节作为金黄色葡萄球菌调节基因表达的一种机制正在获得认可，但调控转录物降解改变的因素尚不清楚。在这方面，我们之前发现138种mRNA物种，包括编码毒力因子蛋白A （spa）和胶原结合蛋白（cna）的转录本，在指数期生长时以依赖SarA的方式稳定，这表明SarA直接或间接影响这些转录本的RNA周转特性。在此，我们扩展了sarA在生长的指数晚期和平稳阶段对mRNA周转的影响的表征。结果表明，该位点影响细胞在两个生长阶段的RNA降解特性。此外，利用凝胶迁移率转移试验和RIP-Chip，发现SarA蛋白能够结合mRNA物种，并在体外和细菌细胞内稳定。综上所述，这些结果表明SarA转录后调控金黄色葡萄球菌基因表达的方式涉及结合并因此改变靶转录物的mRNA周转特性。
The modulation of mRNA turnover is gaining recognition as a mechanism by which Staphylococcus aureus regulates gene expression, but the factors that orchestrate alterations in transcript degradation are poorly understood. In that regard, we previously found that 138 mRNA species, including transcripts coding for the virulence factors protein A (spa) and collagen-binding protein (cna), are stabilized in a sarA-dependent manner during exponential phase growth, suggesting that SarA directly or indirectly affects the RNA turnover properties of these transcripts. Herein, we expanded our characterization of the effects of sarA on mRNA turnover during late-exponential and stationary phases of growth. Results revealed that the locus affects the RNA degradation properties of cells during both growth phases. Further, using gel mobility shift assays and RIP-Chip, it was found that SarA protein is capable of binding mRNA species that it stabilizes both in vitro and within bacterial cells. Taken together, these results suggest that SarA post-transcriptionally regulates S. aureus gene expression in a manner that involves binding to and consequently altering the mRNA turnover properties of target transcripts.
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影响因子：
11.4
作者：
MORFELDT, E;TAYLOR, D;ARVIDSON, S
通讯作者：
ARVIDSON, S
影响因子：
168.9
作者：
DeLeo, Frank R.;Otto, Michael;Kreiswirth, Barry N.;Chambers, Henry F.
通讯作者：
Chambers, Henry F.
影响因子：
3.1
作者：
DEAZAVEDO, JCS;FOSTER, TJ;NOVICK, RP
通讯作者：
NOVICK, RP
影响因子：
4.8
作者：
Chien, YT;Manna, AC;Cheung, AL
通讯作者：
Cheung, AL
影响因子：
5.8
作者：
Carneiro, CRW;Postol, E;Brentani, RR
通讯作者：
Brentani, RR