Amperometric Vitamin C Biosensor Based on the Immobilization of Ascorbate Oxidase into the Biocompatible Sandwich-Type Composite Film
Amperometric Vitamin C Biosensor Based on the Immobilization of Ascorbate Oxidase into the Biocompatible Sandwich-Type Composite Film
复制标题
基于生物相容性三明治型复合膜固定抗坏血酸氧化酶的电流式维生素 C 生物传感器
DOI：
10.1007/s12010-012-9711-y
复制
发表时间：
2012-05
影响因子：
3
通讯作者：
He, Haohua
中科院分区：
文献类型：
--
作者：
Wen, Yangping;Xu, Jingkun;Liu, Ming;Li, Dong;He, Haohua
将生物活性大分子抗坏血酸氧化酶（AO）成功地固定在生物相容性良好的复合膜上，制备了维生素C（VC）生物传感器，并对市售果汁中的VC含量进行了安培法测定。制备了具有生物相容性和导电性的聚（3，4-乙撑二氧噻吩）复合膜和高稳定性和高选择性的多壁碳纳米管-Nafion复合膜作为生物传感器的内、外膜。AO分子被固定在这两个复合膜之间。该生物传感器对VC的氧化具有良好的电催化性能，电流响应快，工作电位低，灵敏度高，线性范围宽，检测限低。提出了该传感器的工作机理，并对其动力学进行了讨论。此外，还对所制备的生物传感器的特异性、重现性和可行性进行了评价。对市售果汁中维生素C的测定结果表明，该传感器可用于农产品中维生素C的电化学检测。内外膜为生物活性物质的固定化提供了一个很有前途的平台。
Ascorbate oxidase (AO), a biologically active macromolecule, was successfully immobilized into a biocompatible sandwich-type composite film for developing the vitamin C (VC) biosensor, and the content of VC in commercial juices was amperometrically determined. The biocompatible and conducting poly(3,4-ethylenedioxythiophene) composite film and highly stable and selective multiwalled carbon nanotubes –Nafion composite film were prepared as inner and outer films of biosensor. AO molecules were immobilized between these two composite films. The as-fabricated biosensor displayed an excellent bioelectrocatalytic performance towards the oxidation of VC, a fast current response, a low working potential, a high sensitivity, a wide linear range, and a low detection limit. Moreover, the working mechanism of the biosensor was proposed, and its kinetics was also discussed. In addition, the specificity, reproducibility, and feasibility of the as-fabricated biosensor were also evaluated. Good results of the VC determination in commercial juices indicated that the as-fabricated biosensor was a potential candidate for the electrochemical determination of VC in agricultural crops. Inner and outer films provided a promising platform for the immobilization of biologically active species.
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DOI：
10.1016/b978-012373738-0.50017-9
发表时间：
2008-01-01
期刊：
ELECTROCHEMICAL SENSORS, BIOSENSORS AND THEIR BIOMEDICAL APPLICATIONS
影响因子：
--
作者：
Feng, Manliang;Han, Heyou;Tachikawa, Hiroyasu
通讯作者：
Tachikawa, Hiroyasu
离子液体微乳液：一种新的电聚合介质
影响因子：
4.5
作者：
Zheng, Liqiang;Xu, Jingkun;Dong, Bin;Zhang, Shaohua
通讯作者：
Zhang, Shaohua
DOI：
10.3109/10731191003785588
发表时间：
2010-07
期刊：
Artificial Cells, Blood Substitutes, and Biotechnology
影响因子：
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作者：
M. Sezgintürk;H. Koca;Yavuz Selim Özben;E. Dinçkaya
通讯作者：
M. Sezgintürk;H. Koca;Yavuz Selim Özben;E. Dinçkaya
影响因子：
4.5
作者：
V. Serafín;L. Agüí;P. Yáñez‐Sedeño;J. Pingarrón
通讯作者：
V. Serafín;L. Agüí;P. Yáñez‐Sedeño;J. Pingarrón
影响因子：
29.4
作者：
P. Strohriegl;J. Gražulevičius
通讯作者：
P. Strohriegl;J. Gražulevičius