Biofilm Formation of Streptococcus equi ssp. zooepidemicus and Comparative Proteomic Analysis of Biofilm and Planktonic Cells
Biofilm Formation of Streptococcus equi ssp. zooepidemicus and Comparative Proteomic Analysis of Biofilm and Planktonic Cells
复制标题
马链球菌亚种生物膜的形成。
DOI：
10.1007/s00284-014-0574-z
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发表时间：
2014-04
影响因子：
2.6
通讯作者：
Lu, Cheng-ping
中科院分区：
文献类型：
--
作者：
Zheng, Jun-xi;Yang, Yong-chun;Fan, Hong-jie;Lu, Cheng-ping
马链球菌亚种动物寄生虫（SEZ）引起许多物种（包括猪、马和人）中的多种感染。生物膜的形成对于发病机制是必不可少的，并且抵抗抗生素治疗的能力导致难以治疗和持续的感染。然而，经济特区形成生物膜的能力尚不清楚。此外，SEZ生物膜形成的机制及其属性知之甚少。在这项研究中，扫描电子显微镜（SEM）表明，SEZ菌株ATCC 35246形成生物膜，包括一个厚的，不均匀的层与团块上的盖玻片孵育24小时。此外，我们使用了一个二维凝胶电泳（2-DE）为基础的方法来表征差异表达的蛋白质在经济特区生物膜相比，他们的同源性。结果显示，存在24个不同强度的蛋白质点，其中13个上调，11个下调，在SEZ生物膜相比，去甲肾上腺素对照。在生物膜形成过程中表达的蛋白质大多与代谢、粘附和应激条件有关。这些观察有助于我们了解经济特区生物膜的生活方式，这可能会导致更有效的措施来控制持续的经济特区感染。
Streptococcus equi ssp. zooepidemicus (SEZ) is responsible for a wide variety of infections in many species, including pigs, horses and humans. Biofilm formation is essential for pathogenesis, and the ability to resist antibiotic treatment results in difficult-to-treat and persistent infections. However, the ability of SEZ to form biofilms is unclear. Furthermore, the mechanisms underlying SEZ biofilm formation and their attributes are poorly understood. In this study, scanning electron microscopy (SEM) demonstrated that SEZ strain ATCC35246 formed biofilms comprising a thick, heterogeneous layer with clumps on the coverslips when incubated for 24 h. In addition, we used a two-dimensional gel electrophoresis (2-DE) based approach to characterize differentially expressed protein in SEZ biofilms compared with their planktonic counterparts. The results revealed the existence of 24 protein spots of varying intensities, 13 of which were upregulated and 11 were downregulated in the SEZ biofilm compared with the planktonic controls. Most of proteins expressed during biofilm formation were associated with metabolism, adhesion, and stress conditions. These observations contribute to our understanding of the SEZ biofilm lifestyle, which may lead to more effective measures to control persistent SEZ infections.
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影响因子：
5
作者：
Ren, D;Bedzyk, LA;Wood, TK
通讯作者：
Wood, TK
影响因子：
2.1
作者：
He, Xinlong;Ahn, Juhee
通讯作者：
Ahn, Juhee
DOI：
10.1099/00221287-65-3-351
发表时间：
1971-03
期刊：
Journal of general microbiology
影响因子：
--
作者：
R. Williams;S. A. Sadler
通讯作者：
R. Williams;S. A. Sadler
影响因子：
3.2
作者：
Xia-wei Jiang;Shuang Wang;Hong Cheng;Y. Huo;Xin-Qi Zhang;Xu-fen Zhu;Xifang Han;Peixiang Ni;
通讯作者：
Xia-wei Jiang;Shuang Wang;Hong Cheng;Y. Huo;Xin-Qi Zhang;Xu-fen Zhu;Xifang Han;Peixiang Ni;
影响因子：
4.4
作者：
Jeffrey Perkel
通讯作者：
Jeffrey Perkel