Central myelin gene expression during postnatal development in rats exposed to nicotine gestationally.
Central myelin gene expression during postnatal development in rats exposed to nicotine gestationally.
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DOI：
10.1016/j.neulet.2013.08.012
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发表时间：
2013-10-11
影响因子：
2.5
通讯作者：
Li MD
中科院分区：
文献类型：
--
作者：
Cao J;Dwyer JB;Gautier NM;Leslie FM;Li MD
中枢神经系统髓鞘基因的异常表达与许多精神疾病有关，包括精神障碍和药物成瘾。我们之前已经证明，出生前暴露于尼古丁，香烟烟雾中的主要精神活性成分，改变了青春期大鼠中枢神经系统髓鞘基因的表达。为了检验这种效应是否对青少年特有，我们检测了青少年和成年人中枢神经系统中髓鞘基因的表达。用微量渗透压泵给妊娠SD大鼠注射尼古丁(3 mg/kg/d；GN)或生理盐水(GS)。仔鼠出生后20~21天(幼龄)、35~36天(青少年)或59~60天(成年)处死雌雄。解剖前额叶皮质(PFC)、尾壳核(CPU)和伏隔核(NAC)3个边缘脑区。用定量RT-PCR方法检测髓鞘主要成分编码基因的表达。我们发现，GN改变了不同脑区和性别的青少年的髓鞘基因表达。这种改变的模式与在青少年中观察到的不同。虽然这些基因在成年男性中正常表达，但我们观察到GN处理的成年女性表达减少，特别是在CPU中。因此，GN改变了髓鞘基因在出生后发育和成年期的表达。对青少年的影响与其他年龄段截然不同，这与青春期许多精神障碍的独特症状有关。
Abnormal myelin gene expression in the central nervous system (CNS) is associated with many mental illnesses, including psychiatric disorders and drug addiction. We have previously shown that prenatal exposure to nicotine, the major psychoactive component in cigarette smoke, alters myelin gene expression in the CNS of adolescent rats. To examine whether this effect is specific for adolescents, we examined myelin gene expression in the CNS of juveniles and adults. Pregnant Sprague-Dawley rats were treated with nicotine (3 mg/kg/day; GN) or saline (GS) via osmotic mini pumps from gestational days 4 to 18. Both male and female offspring were sacrificed at postnatal day P20-21 (juveniles), P35-36 (adolescents), or P59-60 (adults). Three limbic brain regions, the prefrontal cortex (PFC), caudate putamen (CPu), and nucleus accumbens (NAc), were dissected. The expression of genes encoding major myelin components was evaluated using quantitative RT-PCR. We found that GN altered myelin gene expression in juveniles with brain region and sex differences. The pattern of alteration was different from that observed in adolescents. Although these genes were expressed normally in male adults, we observed decreased expression in GN-treated female adults, especially in the CPu. Thus, GN altered myelin gene expression throughout postnatal development and adulthood. The effect on adolescents was quite different from that at other ages, which correlated with the unique symptoms of many psychiatric disorders during adolescence.
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影响因子：
3.4
作者：
Franke, Ryan M.;Belluzzi, James D.;Leslie, Frances M.
通讯作者：
Leslie, Frances M.
影响因子：
30.8
作者：
Lappe-Siefke, C;Goebbels, S;Nave, KA
通讯作者：
Nave, KA
影响因子：
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作者：
Hursh, JB
通讯作者：
Hursh, JB
影响因子：
3.4
作者：
Bora, Emre;Yuecel, Murat;Lubman, Dan I.
通讯作者：
Lubman, Dan I.
DOI：
10.1073/pnas.1121251109
发表时间：
2012-10-16
影响因子：
11.1
作者：
Kolb, Bryan;Mychasiuk, Richelle;Gibb, Robbin
通讯作者：
Gibb, Robbin