Transcriptomic analysis of three Veillonella spp. present in carious dentine and in the saliva of caries-free individuals.
Transcriptomic analysis of three Veillonella spp. present in carious dentine and in the saliva of caries-free individuals.
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DOI：
10.3389/fcimb.2015.00025
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发表时间：
2015
影响因子：
5.7
通讯作者：
Beighton D
中科院分区：
文献类型：
--
作者：
Do T;Sheehy EC;Mulli T;Hughes F;Beighton D
细孔菌是所有口腔生物膜中发现的主要细菌。在本研究中，采用超转录组学方法研究了三种口腔细孔菌（细小细孔菌、异斑细孔菌和异型细孔菌）在无龋齿志愿者的全刺激唾液和龋齿病变中的基因表达水平（每组11例）。在病变中，被分配给小苗弧菌的reads比例最大，在各种样品中表达水平最高的基因是那些编码膜运输系统的基因。所有三种细孔菌都增加了与乳酸和琥珀酸盐分解代谢有关的基因的表达，特别是L(+)-酒石酸脱水酶的α -亚基和β -亚基（EC 4.2.1.32）。组氨酸生物合成途径在小叶弧菌中的表达也显著增加，表明细胞内组氨酸水平较高，可以提供细胞内缓冲能力，从而有助于在酸性环境中生存。各种其他系统，如钾吸收系统也被上调，这可能有助于小叶弧菌在龋齿病变中的存活和增殖。
Veillonella spp. are predominant bacteria found in all oral biofilms. In this study, a metatranscriptomic approach was used to investigate the gene expression levels of three oral Veillonella spp. (V. parvula, V. dispar and V. atypica) in whole stimulated saliva from caries-free volunteers and in carious lesions (n = 11 for each group). In the lesions the greatest proportion of reads were assigned to V. parvula and genes with the highest level of expression in carious samples were those coding for membrane transport systems. All three Veillonella spp. increased expression of genes involved in the catabolism of lactate and succinate, notably the alpha- and beta-subunits of L(+)-tartrate dehydratase (EC 4.2.1.32). There was also significantly increased expression of histidine biosynthesis pathway in V. parvula, suggesting higher intra-cellular levels of histidine that could provide intra-cellular buffering capacity and, therefore, assist survival in the acidic environment. Various other systems such as potassium uptake systems were also up regulated that may aid in the survival and proliferation of V. parvula in carious lesions.
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影响因子：
11
作者：
Duran-Pinedo, Ana E.;Chen, Tsute;Frias-Lopez, Jorge
通讯作者：
Frias-Lopez, Jorge
影响因子：
6.4
作者：
Jorth P;Turner KH;Gumus P;Nizam N;Buduneli N;Whiteley M
通讯作者：
Whiteley M
DOI：
10.1099/ijs.0.02952-0
发表时间：
2004-07-01
影响因子：
2.8
作者：
Jumas-Bilak, E;Carlier, JP;Marchandin, H
通讯作者：
Marchandin, H
影响因子：
--
作者：
Beighton, D.;Clark, D.;Do, T.
通讯作者：
Do, T.
影响因子：
4.4
作者：
Brockmann-Gretza O;Kalinowski J
通讯作者：
Kalinowski J