Expression and Distribution of the Guanine Nucleotide-binding Protein Subunit Alpha-s in Mice Skin Tissues and Its Association with White and Black Coat Colors.
Expression and Distribution of the Guanine Nucleotide-binding Protein Subunit Alpha-s in Mice Skin Tissues and Its Association with White and Black Coat Colors.
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鸟嘌呤核苷酸结合蛋白亚基α-s在小鼠皮肤组织中的表达和分布及其与白色和黑色毛色的关系。
DOI：
10.5713/ajas.15.0711
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发表时间：
2016-10
期刊：
影响因子：
--
通讯作者：
Pang Q
中科院分区：
文献类型：
--
作者：
Yin Z;Zhao X;Wang Z;Li Z;Bai R;Yang S;Zhao M;Pang Q
鸟嘌呤核苷酸结合蛋白亚基α s（Gnαs）是G蛋白偶联信号通路的一个小亚基，参与毛色的形成。采用实时荧光定量PCR（qPCR）、western blot和免疫组织化学方法检测Gnαs在小鼠白色和黑色皮肤组织中的表达水平和分布，探讨小鼠毛色形成的机制。qPCR和western blot结果表明，Gnαs在黑鼠中的表达水平显著高于白色鼠，且在黑鼠和白鼠中的表达水平相同。免疫组化显示Gnαs主要分布于毛囊的根鞘和毛乳头。结果表明，Gnαs基因在白色和黑色皮肤组织中均有表达，但在两种肤色中的表达量不同。因此，Gnαs可能参与了小鼠毛色的形成。
Guanine nucleotide-binding protein subunit alpha-s (Gnαs) is a small subunit of the G protein-couple signaling pathway, which is involved in the formation of coat color. The expression level and distribution of Gnαs were detected by quantitative real-time-polymerase chain reaction (qPCR), western blot, and immunohistochemistry to investigate the underlying mechanisms of coat color in white and black skin tissues of mice. qPCR and western blot results suggested that Gnαs was expressed at significantly higher levels in black mice compared with that of white mice, and transcripts and protein possessed the same expression in both colors. Immunohistochemistry demonstrated Gnαs staining in the root sheath and dermal papilla in hair follicle of mice skins. The results indicated that the Gnαs gene was expressed in both white and black skin tissues, and the expression level of Gnαs in the two types of color was different. Therefore, Gnαs may be involved in the coat color formation in mice.
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影响因子：
3.7
作者：
Dorshorst B;Henegar C;Liao X;Sällman Almén M;Rubin CJ;Ito S;Wakamatsu K;Stothard P;Van Doormaal B;Plastow G;Barsh GS;Andersson L
通讯作者：
Andersson L
影响因子：
2.4
作者：
Vage, D. I.;Nieminen, M.;Roed, K. H.
通讯作者：
Roed, K. H.
影响因子：
4.4
作者：
Tian X;Jiang J;Fan R;Wang H;Meng X;He X;He J;Li H;Geng J;Yu X;Song Y;Zhang D;Yao J;Smith GW;Dong C
通讯作者：
Dong C
DOI：
10.5713/ajas.2002.1395
发表时间：
2002-10-01
期刊：
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES
影响因子：
--
作者：
Jiang, YL;Fan, XZ;Du, LX
通讯作者：
Du, LX
影响因子：
3.1
作者：
Lai, Fenju;Ren, Jun;Huang, Lusheng
通讯作者：
Huang, Lusheng