MoWhi2 regulates appressorium formation and pathogenicity via the MoTor signalling pathway in Magnaporthe oryzae.
MoWhi2 regulates appressorium formation and pathogenicity via the MoTor signalling pathway in Magnaporthe oryzae.
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MoWhi2 通过 MoTor 信号通路调节稻瘟病菌的附着胞形成和致病性
DOI：
10.1111/mpp.13074
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发表时间：
2021-08
影响因子：
4.9
通讯作者：
Kou Y
中科院分区：
文献类型：
--
作者：
Shi H;Meng S;Qiu J;Wang C;Shu Y;Luo C;Kou Y
摘要稻瘟病是稻瘟病的主要病原，严重威胁着食品生产的安全。附着胞的形成是水稻稻瘟病菌成功侵染的关键步骤之一，了解附着胞的形成机制有助于制定有效的稻瘟病防治策略。在本研究中，我们鉴定了酿酒酵母（Saccharomyces cerevisiae） wh2 （Whisky2）的同源物mowh2是M. oryzae中控制附着胞形成的重要调节因子。当mowh2被破坏时，一个分生孢子形成多个附着胞，致病性显著降低。通过酵母双杂交筛选实验，确定了一种假定的磷酸酶MoPsr1与mowh2相互作用。敲除突变体ΔMopsr1表现出与ΔMowhi2菌株相似的表型。与野生型（WT）相比，ΔMowhi2和ΔMopsr1突变体都可以在亲水表面形成附着胞，cAMP水平升高。当雷帕霉素（TOR）抑制剂靶物雷帕霉素存在时，ΔMowhi2和ΔMopsr1的分生孢子与WT类似，每个分生孢子形成一个附着胞。此外，与WT相比，MoTOR及MoTOR信号激活标记基因MoRS3的表达水平均有所升高，提示对MoTOR信号通路的不当激活是ΔMowhi2和ΔMopsr1菌株附着胞形成缺陷的重要原因之一。我们的研究结果揭示了moryzae中mowh2和MoPsr1通过调节cAMP水平和运动信号激活介导的附着胞发育和致病性。
Abstract Magnaporthe oryzae causes rice blast disease, which seriously threatens the safety of food production. Understanding the mechanism of appressorium formation, which is one of the key steps for successful infection by M. oryzae, is helpful to formulate effective control strategies of rice blast. In this study, we identified MoWhi2, the homolog of Saccharomyces cerevisiae Whi2 (Whisky2), as an important regulator that controls appressorium formation in M. oryzae. When MoWHI2 was disrupted, multiple appressoria were formed by one conidium and pathogenicity was significantly reduced. A putative phosphatase, MoPsr1, was identified to interact with MoWhi2 using a yeast two‐hybridization screening assay. The knockout mutant ΔMopsr1 displayed similar phenotypes to the ΔMowhi2 strain. Both the ΔMowhi2 and ΔMopsr1 mutants could form appressoria on a hydrophilic surface with cAMP levels increasing in comparison with the wild type (WT). The conidia of ΔMowhi2 and ΔMopsr1 formed a single appressorium per conidium, similar to WT, when the target of rapamycin (TOR) inhibitor rapamycin was present. In addition, compared with WT, the expression levels of MoTOR and the MoTor signalling activation marker gene MoRS3 were increased, suggesting that inappropriate activation of the MoTor signalling pathway is one of the important reasons for the defects in appressorium formation in the ΔMowhi2 and ΔMopsr1 strains. Our results provide insights into MoWhi2 and MoPsr1‐mediated appressorium development and pathogenicity by regulating cAMP levels and the activation of MoTor signalling in M. oryzae.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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