Tpc1 is an important Zn(II)2Cys6 transcriptional regulator required for polarized growth and virulence in the rice blast fungus.
Tpc1 is an important Zn(II)2Cys6 transcriptional regulator required for polarized growth and virulence in the rice blast fungus.
复制标题
DOI：
10.1371/journal.ppat.1006516
复制
发表时间：
2017-07
期刊：
影响因子：
6.7
通讯作者：
Sesma A
中科院分区：
文献类型：
--
作者：
Galhano R;Illana A;Ryder LS;Rodríguez-Romero J;Demuez M;Badaruddin M;Martinez-Rocha AL;Soanes DM;Studholme DJ;Talbot NJ;Sesma A
极性的建立是病原真菌的关键过程，介导感染相关的形态发生和宿主组织侵袭。在这里，我们报告了 TPC1（极性控制转录因子 1）的鉴定，它调节稻瘟病菌 Magnaporthe oryzae 的侵入性极化生长。 TPC1 编码真菌 Zn(II)2Cys6 家族的推定转录因子，为丝状真菌所独有。 Tpc1 缺陷突变体在分生孢子发生、感染相关的自噬、糖原和脂质代谢以及植物组织定植方面表现出严重缺陷。通过追踪肌动蛋白结合蛋白、septin-5 和自噬体成分，我们发现 Tpc1 在附着胞介导的植物渗透过程中调节细胞骨架动力学和感染相关的自噬。我们发现Tpc1与Mst12相互作用并调节其DNA结合活性，而Tpc1核定位也依赖于MAP激酶Pmk1，这与Tpc1参与该信号通路一致，这对于附着胞的发育至关重要。重要的是，Tpc1 通过与 Mst12 相互作用直接调节 NOXD 表达，即真菌 NADPH 氧化酶复合物的 p22phox 亚基。因此，Tpc1 通过在附着胞再极化过程中调节 NADPH 氧化酶复合物来控制皮质 F-肌动蛋白的空间和时间调节。因此，Tpc1 是丝状真菌的核心发育调节因子，将活性氧的调节合成和 Pmk1 途径与宿主入侵期间的极性控制联系起来。细胞极性是丝状真菌生长和发病机制的固有特征。在这项研究中，我们鉴定了稻瘟病菌 Magnaporthe oryzae 中真菌极性生长和毒力所需的基因。该基因被命名为 TPC1（极性控制转录因子 1）。 Tpc1 蛋白属于真菌 Zn(II)2Cys6 双核簇家族。这种 DNA 结合基序仅存在于真菌界。我们已经表征了米霉中与 Tpc1 缺乏相关的缺陷。我们证明 Tpc1 参与极化生长和毒力。米霉 Δtpc1 突变体表现出糖原和脂质代谢的延迟，以及调节附着胞介导的米霉植物感染的感染相关自噬过程的延迟。腐生真菌粗糙脉孢菌 (Neurospora crassa) 含有参与营养生长和持续尖端伸长的 Tpc1 同源物 (NcTpc1)，表明 Tpc1 样蛋白是丝状真菌极化生长和发育的核心调节因子。比较转录组分析使我们能够识别米霉中受 Tpc1 调节的基因，包括真菌 NADPH 复合物的重要组成部分 NoxD。值得注意的是，Tpc1 与 Mst12 相互作用，Mst12 是附着胞发育所必需的 Pmk1 信号通路的一个组成部分，并调节 Mst12 与 NOXD 启动子区域的结合亲和力。我们得出的结论是，Tpc1 是米霉极性的关键调节因子，可调节生长、自噬和 septin 介导的 F-肌动蛋白细胞骨架的重新定向，以促进植物细胞侵袭。
The establishment of polarity is a critical process in pathogenic fungi, mediating infection-related morphogenesis and host tissue invasion. Here, we report the identification of TPC1 (Transcription factor for Polarity Control 1), which regulates invasive polarized growth in the rice blast fungus Magnaporthe oryzae. TPC1 encodes a putative transcription factor of the fungal Zn(II)2Cys6 family, exclusive to filamentous fungi. Tpc1-deficient mutants show severe defects in conidiogenesis, infection-associated autophagy, glycogen and lipid metabolism, and plant tissue colonisation. By tracking actin-binding proteins, septin-5 and autophagosome components, we show that Tpc1 regulates cytoskeletal dynamics and infection-associated autophagy during appressorium-mediated plant penetration. We found that Tpc1 interacts with Mst12 and modulates its DNA-binding activity, while Tpc1 nuclear localisation also depends on the MAP kinase Pmk1, consistent with the involvement of Tpc1 in this signalling pathway, which is critical for appressorium development. Importantly, Tpc1 directly regulates NOXD expression, the p22phox subunit of the fungal NADPH oxidase complex via an interaction with Mst12. Tpc1 therefore controls spatial and temporal regulation of cortical F-actin through regulation of the NADPH oxidase complex during appressorium re-polarisation. Consequently, Tpc1 is a core developmental regulator in filamentous fungi, linking the regulated synthesis of reactive oxygen species and the Pmk1 pathway, with polarity control during host invasion. Cellular polarity is an intrinsic feature of filamentous fungal growth and pathogenesis. In this study, we identified a gene required for fungal polar growth and virulence in the rice blast fungus Magnaporthe oryzae. This gene has been named TPC1 (Transcription factor for Polarity Control 1). The Tpc1 protein belongs to the fungal Zn(II)2Cys6 binuclear cluster family. This DNA-binding motif is present exclusively in the fungal kingdom. We have characterised defects associated with lack of Tpc1 in M. oryzae. We show that Tpc1 is involved in polarised growth and virulence. The M. oryzae Δtpc1 mutant shows a delay in glycogen and lipid metabolism, and infection-associated autophagy–processes that regulate appressorium-mediated M. oryzae plant infection. The saprophytic fungus Neurospora crassa contains a Tpc1 homolog (NcTpc1) involved in vegetative growth and sustained tip elongation, suggesting that Tpc1-like proteins act as core regulators of polarised growth and development in filamentous fungi. A comparative transcriptome analysis has allowed us to identify genes regulated by Tpc1 in M. oryzae including NoxD, an important component of the fungal NADPH complex. Significantly, Tpc1 interacts with Mst12, a component of the Pmk1 signalling pathway essential for appressorium development, and modulates Mst12 binding affinity to NOXD promoter region. We conclude that Tpc1 is a key regulator of polarity in M. oryzae that regulates growth, autophagy and septin-mediated reorientation of the F-actin cytoskeleton to facilitate plant cell invasion.
登录
查看更多内容
影响因子：
3
作者：
Battaglia, Evy;Klaubauf, Sylvia;de Vries, Ronald P.
通讯作者：
de Vries, Ronald P.
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
2.7
作者：
Collins, Tony J.
通讯作者：
Collins, Tony J.
影响因子：
--
作者：
Fu, Ci;Iyer, Priyadarshini;Free, Stephen J.
通讯作者：
Free, Stephen J.
影响因子：
56.9
作者：
Dagdas, Yasin F.;Yoshino, Kae;Talbot, Nicholas J.
通讯作者：
Talbot, Nicholas J.