Use of autologous blood-derived endothelial progenitor cells at point-of-care to protect against implant thrombosis in a large animal model.
Use of autologous blood-derived endothelial progenitor cells at point-of-care to protect against implant thrombosis in a large animal model.
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DOI：
10.1016/j.biomaterials.2011.07.066
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发表时间：
2011-11
期刊：
影响因子：
14
通讯作者：
Achneck, Hardean E.
中科院分区：
文献类型：
--
作者：
Jantzen, Alexandra E.;Lane, Whitney O.;Gage, Shawn M.;Jamiolkowski, Ryan M.;Haseltine, Justin M.;Galinat, Lauren J.;Lin, Fu-Hsiung;Lawson, Jeffrey H.;Truskey, George A.;Achneck, Hardean E.
钛（Ti）通常用于许多心血管装置，例如作为镍钛诺支架的组成部分，体内和体外机械循环辅助装置，但与血栓栓塞形成的风险相关。我们建议通过在Ti血液接触表面内衬来自自体外周血的晚期生长内皮祖细胞（EPCs）来解决这个问题，之前已经证明这些EPCs在生理剪切应力下粘附并在Ti上生长，并在体外流动条件下功能适应其环境。在手术室的护理点，将自体荧光标记的猪EPCs播种到Ti管上30分钟，然后植入猪下腔静脉的促血栓形成环境（n = 8）。3天后，取出钛管，拆卸，对血接触表面进行成像。一项盲法分析发现，所有4个细胞种子植入物均无凝块，而没有EPCs的4个对照组要么完全闭塞，要么部分血栓形成。预先标记的EPCs在所有4个细胞种子植入物中均已扩散并存在，而在对照植入物中未观察到内皮细胞。这些结果表明，生长较晚的自体EPCs是一种很有希望的Ti衬里植入物来源，可以减少体内血栓形成。
Titanium (Ti) is commonly utilized in many cardiovascular devices, e.g. as a component of Nitinol stents, intra- and extracorporeal mechanical circulatory assist devices, but is associated with the risk of thromboemboli formation. We propose to solve this problem by lining the Ti blood-contacting surfaces with autologous peripheral blood-derived late outgrowth endothelial progenitor cells (EPCs) after having previously demonstrated that these EPCs adhere to and grow on Ti under physiological shear stresses and functionally adapt to their environment under flow conditions ex vivo. Autologous fluorescently-labeled porcine EPCs were seeded at the point-of-care in the operating room onto Ti tubes for 30 minutes and implanted into the pro-thrombotic environment of the inferior vena cava of swine (n = 8). After 3 days, Ti tubes were explanted, disassembled, and the blood-contacting surface was imaged. A blinded analysis found all 4 cell-seeded implants to be free of clot, whereas 4 controls without EPCs were either entirely occluded or partially thrombosed. Pre-labeled EPCs had spread and were present on all 4 cell-seeded implants while no endothelial cells were observed on control implants. These results suggest that late outgrowth autologous EPCs represent a promising source of lining Ti implants to reduce thrombosis in vivo.
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影响因子：
2.5
作者：
Achneck, Hardean E.;Serpe, Michael J.;Jamiolkowski, Ryan M.;Eibest, Leslie M.;Craig, Stephen L.;Lawson, Jeffrey H.
通讯作者：
Lawson, Jeffrey H.
影响因子：
6.7
作者：
Kang, C;Bonneau, M;Drouet, L
通讯作者：
Drouet, L
影响因子：
4.8
作者：
Forbes, TJ;Rosenthal, GL;Feltes, TF
通讯作者：
Feltes, TF
影响因子：
1.3
作者：
SELDINGER, SI
通讯作者：
SELDINGER, SI
DOI：
10.1161/01.atv.13.2.197
发表时间：
1993-02-01
期刊：
ARTERIOSCLEROSIS AND THROMBOSIS
影响因子：
--
作者：
LI, H;CYBULSKY, MI;LIBBY, P
通讯作者：
LIBBY, P