Ulex Europaeus Agglutinin-I-Based Magnetic Isolation for the Efficient and Specific Capture of SW480 Circulating Colorectal Tumor Cells.
Ulex Europaeus Agglutinin-I-Based Magnetic Isolation for the Efficient and Specific Capture of SW480 Circulating Colorectal Tumor Cells.
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基于 Ulex Europaeus 凝集素 I 的磁分离可高效、特异性捕获 SW480 循环结直肠肿瘤细胞
DOI：
10.1021/acsomega.2c03702
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发表时间：
2022-08-30
期刊：
影响因子：
4.1
通讯作者：
Wang, Zhenxin
中科院分区：
文献类型：
--
作者：
Tian, Rongrong;Li, Xiaodong;Zhang, Hua;Ma, Lina;Zhang, Huimao;Wang, Zhenxin
从患者外周血中高效特异地捕获循环肿瘤细胞（CTC）对于精确的癌症诊断和癌症治疗具有重要价值。凝集素作为一种精细的靶向分子，由于癌细胞膜上分子的异常糖基化以及凝集素与糖缀合物的特异性结合，能够特异性地识别癌细胞。在本文中，开发了基于荆豆凝集素-I（UEA-I）的磁性分离策略，以有效且特异性地从结直肠癌（CRC）患者的外周血中捕获α-1，2-岩藻糖过表达的CTC。使用UEA-I修饰的Fe 3 O 4磁珠（称为MB-UEA-I），高达94%和89%的靶细胞（即，分别从加标细胞的完全细胞培养基和全血中捕获SW 480 CRC细胞）。超过90%的捕获细胞显示出良好的活力和增殖能力，而不从MB-UEA-I上脱落。结合三色免疫细胞化学（ICC）鉴定，MB-UEA-I已成功用于从CRC患者外周血中捕获CTC。实验结果表明CTC表征与肿瘤转移之间的相关性。具体而言，MB-UEA-I可通过在用作液体活检时捕获CTC来应用于筛查早期CRC。所提出的工作提供了一个新的见解，开发成本效益的凝集素功能化的方法，用于生物医学应用。
The efficient and specific capture of circulating tumor cells (CTCs) from patients’ peripheral blood is of significant value in precise cancer diagnosis and cancer therapy. As fine targeting molecules, lectins can recognize cancer cells specifically due to the abnormal glycosylation of molecules on the cancer cell membrane and the specific binding of lectin with glycoconjugates. Herein, a Ulex europaeus agglutinin-I (UEA-I)-based magnetic isolation strategy was developed to efficiently and specifically capture α-1,2-fucose overexpression CTCs from colorectal cancer (CRC) patients’ peripheral blood. Using UEA-I-modified Fe3O4 magnetic beads (termed MB-UEA-I), up to 94 and 89% of target cells (i.e., SW480 CRC cells) were captured from the cell spiking complete cell culture medium and whole blood, respectively. More than 90% of captured cells show good viability and proliferation ability without detaching from MB-UEA-I. In combination with three-color immunocytochemistry (ICC) identification, MB-UEA-I has been successfully used to capture CTCs from CRC patients’ peripheral blood. The experimental results indicate a correlation between CTC characterization and tumor metastasis. Specifically, MB-UEA-I can be applied to screen early CRC by capturing CTCs when served as a liquid biopsy. The presented work offers a new insight into developing cost-effective lectin-functionalized methods for biomedical applications.
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影响因子：
19
作者：
Li, Zhi;Wang, Ganglin;Ma, Nan
通讯作者：
Ma, Nan
影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
DOI：
10.1126/science.1235226
发表时间：
2013-09-13
期刊：
Science (New York, N.Y.)
影响因子：
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作者：
Plaks V;Koopman CD;Werb Z
通讯作者：
Werb Z
天然鱼陷阱式纳米笼，用于无标记捕获循环肿瘤细胞
DOI：
10.1002/advs.202002259
发表时间：
2020-11
期刊：
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
影响因子：
--
作者：
Jiang W;Han L;Yang L;Xu T;He J;Peng R;Liu Z;Zhang C;Yu X;Jia L
通讯作者：
Jia L
影响因子：
8.4
作者：
FIDLER, IJ
通讯作者：
FIDLER, IJ