Phosphoproteomic analysis of the response of maize leaves to drought, heat and their combination stress.
Phosphoproteomic analysis of the response of maize leaves to drought, heat and their combination stress.
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DOI：
10.3389/fpls.2015.00298
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发表时间：
2015
影响因子：
5.6
通讯作者：
Wang W
中科院分区：
文献类型：
--
作者：
Hu X;Wu L;Zhao F;Zhang D;Li N;Zhu G;Li C;Wang W
干旱和高温，特别是干旱和高温的组合，极大地影响了作物产量。许多研究已经描述了植物在干旱或高温胁迫下的转录组、蛋白质组和磷酸蛋白质组的变化。然而，关于作物对复合胁迫响应的磷蛋白组学变化的研究还很少。为了了解玉米对干旱和高温复合胁迫的响应机制，本研究利用多重iTRAQ定量蛋白质组学和LC-MS/MS方法对玉米叶片进行了蛋白质组分析。对五叶期玉米进行干旱、高温或两者联合处理，采集叶片。在全球范围内，高温、干旱和联合胁迫分别显著改变了172、149和144个磷酸肽的磷酸化水平。这些磷酸肽对应于282个蛋白质。其中，23个只对复合胁迫有反应，不能从单一应激源的反应中预测；30个和75个分别对干旱和高温有反应。值得注意的是，19个蛋白质在不同的位置被磷酸化，以响应单一和组合的压力。在鉴定的7个显著丰富的磷酸化基序中，2个在所有胁迫下共同存在，2个在高温和复合胁迫下共同存在，1个在复合胁迫下特有。三种胁迫下，磷蛋白参与的信号转导途径明显不同。对这些磷蛋白及其途径的功能描述可能导致提高作物抗逆性的新目标，这在面对气候变化和非生物胁迫日益普遍的情况下将特别重要。
Drought and heat stress, especially their combination, greatly affect crop production. Many studies have described transcriptome, proteome and phosphoproteome changes in response of plants to drought or heat stress. However, the study about the phosphoproteomic changes in response of crops to the combination stress is scare. To understand the mechanism of maize responses to the drought and heat combination stress, phosphoproteomic analysis was performed on maize leaves by using multiplex iTRAQ-based quantitative proteomic and LC-MS/MS methods. Five-leaf-stage maize was subjected to drought, heat or their combination, and the leaves were collected. Globally, heat, drought and the combined stress significantly changed the phosphorylation levels of 172, 149, and 144 phosphopeptides, respectively. These phosphopeptides corresponded to 282 proteins. Among them, 23 only responded to the combined stress and could not be predicted from their responses to single stressors; 30 and 75 only responded to drought and heat, respectively. Notably, 19 proteins were phosphorylated on different sites in response to the single and combination stresses. Of the seven significantly enriched phosphorylation motifs identified, two were common for all stresses, two were common for heat and the combined stress, and one was specific to the combined stress. The signaling pathways in which the phosphoproteins were involved clearly differed among the three stresses. Functional characterization of the phosphoproteins and the pathways identified here could lead to new targets for the enhancement of crop stress tolerance, which will be particularly important in the face of climate change and the increasing prevalence of abiotic stressors.
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