ram1 gene, encoding a subunit of farnesyltransferase, contributes to growth, antifungal susceptibility to amphotericin B of Aspergillus fumigatus
ram1 gene, encoding a subunit of farnesyltransferase, contributes to growth, antifungal susceptibility to amphotericin B of Aspergillus fumigatus
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ram1 基因编码法尼基转移酶亚基，有助于烟曲霉的生长和对两性霉素 B 的抗真菌敏感性
DOI：
10.1093/mmy/myx002
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发表时间：
2017-11
期刊：
影响因子：
--
通讯作者：
Fang H.
中科院分区：
文献类型：
--
作者：
Qiao J.;Song Y.;Ling Z.;Liu X.;Fang H.
法尼基化是一种重要的翻译后过程，由法尼基转移酶催化。法尼基转移酶的功能先前已在新型隐球菌和白色念珠菌中进行了探究。烟曲霉是一种重要的……（原文最后一个单词“hu”似乎不完整）
Farnesylation, which is catalyzed by farnesyltransferase, is an important posttranslational process. The function of farnesyltransferase has been previously explored in Cryptococcus neoformans and Candida albicans. Aspergillus fumigatus is an important human opportunistic fungal pathogen in immunocompromised patients. Here we discover the role of the ram1 gene, encoding the β-subunit of farnesyltransferase in A. fumigatus, in the fungal growth and antifungal susceptibility. In this study the ram1 gene was disrupted using A. tumefaciens-mediated transformation. The morphology and radial growth of Δram1 were observed. Assays of disk diffusion and broth microdilution were used to determine the susceptibility of Δram1 mutant to commonly clinical used antifungals and the farnesyltransferase inhibitor manumycin A. Deletion of ram1 resulted in a reduced radial growth of A. fumigatus but did not affect the microscopic morphology. Δram1 showed increased susceptibility to the antifungal amphotericin B; however, its susceptibility to azoles and caspofungin was the same to that to the parental strain. Our data indicate that farnesyltransferase is a potential target for design new antifungal agents.
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DOI：
10.3314/jjmm.49.157
发表时间：
2008-07
期刊：
Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology
影响因子：
--
作者：
Jian Qiao;Wei Liu;Ruoyu Li
通讯作者：
Jian Qiao;Wei Liu;Ruoyu Li
DOI：
--
发表时间：
2008-06
影响因子：
--
作者：
Jian Qiao;Wei Liu;Yansen Ma;Z. Wan;Ruo-yu Li
通讯作者：
Jian Qiao;Wei Liu;Yansen Ma;Z. Wan;Ruo-yu Li
影响因子：
2.8
作者：
Tao Xue;C. Nguyen;A. Romans;D. Kontoyiannis;G. May
通讯作者：
Tao Xue;C. Nguyen;A. Romans;D. Kontoyiannis;G. May
影响因子：
5.2
作者：
Perlin, David S;Shor, Erika;Zhao, Yanan
通讯作者：
Zhao, Yanan
影响因子：
2.8
作者：
Song, JL;White, TC
通讯作者：
White, TC