Interactions between estrogen, tamoxifen, octylphenol, and two polychlorinated biphenyls in murine splenocytes.
Interactions between estrogen, tamoxifen, octylphenol, and two polychlorinated biphenyls in murine splenocytes.
复制标题
雌激素、他莫昔芬、辛基苯酚和两种多氯联苯在小鼠脾细胞中的相互作用。
DOI：
10.1016/s0024-3205(99)00346-x
复制
发表时间：
1999
期刊：
影响因子：
6.1
通讯作者：
Blake,CA
中科院分区：
文献类型：
--
作者：
Nair-Menon,JU;Campbell,GT;McCoy,GL;Blake,CA
预先将培养的小鼠脾细胞暴露于17β-雌二醇(E)可保护它们免受外源性雌激素4-叔辛基苯酚(OP)的膜破坏作用。利用从雄性Balb/c小鼠分离的脾细胞，我们测试了(A)异种雌激素2‘，3’，4‘，5’-四氯联苯(PCBOH)或同时具有雌激素和抗雌激素作用的多氯联苯(PCB77)是否会损害细胞膜的完整性，以及(B)E或E受体的另一配体他莫昔芬(TX)是否会保护暴露于这些药物的细胞膜。我们还研究了OP、多氯联苯-羟基和多氯联苯77之间可能的相互作用。脾细胞培养24小时。OP(10−5-10−9M)、PCBOH(10−6−10−16M)或PCB77(10−8−10−12M)浓度对培养的脾细胞膜完整性有明显的剂量效应影响。E浓度高达10−5M或TX高达10−7M均不起作用。脾细胞在含有10−7M的E或TX的培养液中孵育2小时，然后再加入OP、PCBOH或PCB77(最终浓度分别为10−7、10−7或10−8M)，可阻断这种膜破坏效应。加入OP或PCB77后2小时开始用10−7M E孵育脾细胞，或加入OP或PCBOH后2小时开始用10−7M TX培养液孵育脾细胞，均不能阻断OP、PCB77或PCBOH对细胞膜的损伤作用。当使用OP、多氯联苯-羟基或多氯联苯77的各种组合时，没有观察到相互作用。这些数据表明：(A)TX在该系统中的作用类似于E；(B)脾细胞对E或TX的预先反应可以保护它们免受OP、PCBOH或PCB77的潜在细胞毒作用；以及(C)OP、PCBOH和PCB77在其作用中不是相加的。
Prior exposure of cultured murine splenocytes to 17β-estradiol (E) protects them from the membrane disrupting effects of the xenoestrogen 4-tert-octylphenol (OP). Using splenocytes isolated from male Balb/c mice, we tested whether (a) the xenoestrogen, 2′, 3′, 4′, 5′-tetrachloro-4-biphenylol (PCB-OH), or the polychlorinated biphenyl, 3, 3′, 4, 4′-tetrachlorobiphenyl (PCB 77), which displays both estrogenic and anti-estrogenic actions, would compromise the membrane integrity of the cells and (b) E or tamoxifen (TX), another ligand for the E receptor, would protect the membranes of cells exposed to the agents. We also examined possible interactions between OP, PCB-OH, and PCB 77 on the cells. Splenocytes were cultured for 24 hr. Concentrations of OP(10−5-10−9M), PCB-OH (10−6−10−16M), or PCB 77 (10−8−10−12M) significantly compromised the membrane integrity of the cultured splenocytes in a dose response manner. Concentrations of E as high as 10−5M or TX as high as 10−7M were without effect. Incubation of splenocytes in medium containing E or TX at 10−7M for 2 hr prior to the subsequent addition of either OP, PCB-OH or PCB 77 (final concentrations of 10−7, 10−7, or 10−8M, respectively) blocked the membrane disrupting effects. Incubation of splenocytes in medium containing 10−7M E starting 2 hr after the addition of OP or PCB 77 or incubation of splenocytes in medium containing 10−7M TX starting 2 hr after the addition of OP or PCB-OH did not block the damaging effects of OP, PCB 77, or PCB-OH on the cell membranes. No interactions were observed when various combinations of OP, PCB-OH, or PCB 77 were used. These data suggest that: (a) TX acts like E in this system; (b) a prior response of splenocytes to E or TX can protect them from the potential cytotoxic effects of OP, PCB-OH, or PCB 77; and, (c) OP, PCB-OH, and PCB 77 were not additive in their actions.
登录
查看更多内容
多氯联苯对 C57BL/6N 小鼠的免疫抑制活性：Ah 受体激动剂和部分拮抗剂的结构-活性关系。
影响因子：
4.5
作者：
Davis,D;Safe,S
通讯作者：
Safe,S
影响因子：
4.8
作者：
MUELLER, GC;KIM, UH
通讯作者：
KIM, UH
DOI：
10.1016/0162-3109(89)90030-1
发表时间：
1989-07-01
期刊：
IMMUNOPHARMACOLOGY
影响因子：
--
作者：
BARAL, E;KWOK, S;BERCZI, I
通讯作者：
BERCZI, I
雌二醇和他莫昔芬对大鼠混合淋巴细胞反应的影响。
DOI：
10.1016/0162-3109(91)90024-s
发表时间：
1991
期刊：
Immunopharmacology
影响因子：
--
作者：
E. Baral;S. Kwok;I. Bérczi
通讯作者：
I. Bérczi
辛基苯酚对培养的大鼠和小鼠脾细胞的毒性作用。
DOI：
10.1006/taap.1996.0185
发表时间：
1996
期刊：
Toxicology and applied pharmacology.
影响因子：
--
作者：
Nair-Menon,JU;Campbell,GT;Blake,CA
通讯作者：
Blake,CA