Peptidoglycan-dependent NF-κB activation in a small subset of brain octopaminergic neurons controls female oviposition
Peptidoglycan-dependent NF-κB activation in a small subset of brain octopaminergic neurons controls female oviposition
复制标题
一小部分大脑八胺能神经元中肽聚糖依赖性 NF-κB 激活控制雌性产卵
作者：
Ambra Masuzzo;G. Manière;Annelise Viallat;Émilie Avazeri;O. Zugasti;Y. Grosjean;C. Kurz;J. Royet
当面对微生物时，动物会采取降低感染影响的行为。我们先前证明了细菌肽聚糖的内部感应通过某些神经元中的NF-κB途径活化减少果蝇雌性产卵（Kurz等人，2017年）。虽然我们发现神经调质章鱼胺是牵连，涉及的神经元的身份，以及生理机制阻止产卵，仍然未知。在这项研究中，我们确定了少数腹神经索和脑章鱼胺能神经元表达NF-κB通路成分。我们功能性地证明了NF-κB通路在脑中的激活，而不是在腹神经索章鱼胺能神经元中的激活，触发了对感染的产卵下降反应。此外，我们通过钙成像证明，这些神经元的活性可以直接由肽聚糖调节，并且这些细胞不控制其他章鱼胺依赖性行为，如女性接受性。这项研究表明，通过感受肽聚糖，从而激活NF-κB级联，一对脑神经元调节一个特定的章鱼胺依赖性行为，以适应女性的生理状态，以他们的感染状态。
When facing microbes, animals engage in behaviors that lower the impact of the infection. We previously demonstrated that internal sensing of bacterial peptidoglycan reduces Drosophila female oviposition via NF-κB pathway activation in some neurons (Kurz et al., 2017). Although we showed that the neuromodulator octopamine is implicated, the identity of the involved neurons, as well as the physiological mechanism blocking egg-laying, remained unknown. In this study, we identified few ventral nerve cord and brain octopaminergic neurons expressing an NF-κB pathway component. We functionally demonstrated that NF-κB pathway activation in the brain, but not in the ventral nerve cord octopaminergic neurons, triggers an egg-laying drop in response to infection. Furthermore, we demonstrated via calcium imaging that the activity of these neurons can be directly modulated by peptidoglycan and that these cells do not control other octopamine-dependent behaviors such as female receptivity. This study shows that by sensing peptidoglycan and hence activating NF-κB cascade, a couple of brain neurons modulate a specific octopamine-dependent behavior to adapt female physiology status to their infectious state.
登录
查看更多内容
影响因子：
4.5
作者：
Chen J;Reiher W;Hermann-Luibl C;Sellami A;Cognigni P;Kondo S;Helfrich-Förster C;Veenstra JA;Wegener C
通讯作者：
Wegener C
影响因子：
64.5
作者：
Davie K;Janssens J;Koldere D;De Waegeneer M;Pech U;Kreft Ł;Aibar S;Makhzami S;Christiaens V;Bravo González-Blas C;Poovathingal S;Hulselmans G;Spanier KI;Moerman T;Vanspauwen B;Geurs S;Voet T;Lammertyn J;Thienpont B;Liu S;Konstantinides N;Fiers M;Verstreken P;Aerts S
通讯作者：
Aerts S
影响因子：
32.4
作者：
Naitza, S;Rossé, C;Reichhart, JM
通讯作者：
Reichhart, JM
影响因子：
8.8
作者：
Cao X;Kajino-Sakamoto R;Doss A;Aballay A
通讯作者：
Aballay A