Identification of transcription promoter regions from rat mtDNA that are utilized in vivo by the bacterial RNA polymerase.

Identification of transcription promoter regions from rat mtDNA that are utilized in vivo by the bacterial RNA polymerase.
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鉴定大鼠 mtDNA 中被细菌 RNA 聚合酶在体内利用的转录启动子区域。

DOI:
10.1016/0006-291x(90)91588-j
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发表时间:
1990
影响因子:
3.1
通讯作者:
Castora,FJ
Castora,FJ
中科院分区:
生物学4区
文献类型:
--
作者:
Staub,JM;Castora,FJ

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我们已经使用了原核启动子识别载体,pKO-1,分离大鼠线粒体DNA(mtDNA)序列,可以作为细菌的转录启动子。三个假定的启动子含有克隆,扩增到mtDNA探针被确定。通过测量半乳糖激酶活性来定量启动子的强度。这三个启动子分别定位于线粒体DNA的三个不同区域,一个位于16 S rRNA基因的5′端,一个位于ATP酶亚基6基因,最后一个位于细胞色素氧化酶亚基I基因的羧基末端。
We have used a prokaryotic promoter-identification vector, pKO-1, to isolate rat mitochondrial DNA (mtDNA) sequences that can act as bacterial transcription promoters. Three putative promoter-containing clones that hydridized to mtDNA probes were identified. The strength of the promoters was quantitated by measuring galactokinase activity. The three promoters mapped to three distinct regions of the mtDNA-one within the 5′ half of the 16S rRNA gene, one within the ATPase subunit 6 gene, and the last at the carboxy terminal end of the cytochrome oxidase subunit I gene.
DOI: 10.1016/s0021-9258(17)40460-1
发表时间: 1977
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影响因子: --
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DOI: 10.1016/0014-5793(77)80805-3
发表时间: 1977
期刊: FEBS Letters
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作者:
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影响因子: 5.3
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