The venom gland transcriptome of Latrodectus tredecimguttatus revealed by deep sequencing and cDNA library analysis.
The venom gland transcriptome of Latrodectus tredecimguttatus revealed by deep sequencing and cDNA library analysis.
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通过深度测序和 cDNA 文库分析揭示了 Latrodectus tredecimguttatus 的毒腺转录组。
DOI：
10.1371/journal.pone.0081357
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Liang S
中科院分区：
文献类型：
--
作者：
He Q;Duan Z;Yu Y;Liu Z;Liu Z;Liang S
黑斑蛛，俗称黑寡妇蜘蛛，以其危险的咬伤而闻名。虽然它的毒液已经被广泛描述，但关于它的分子组成的一些基本问题仍然没有答案。现有的有限的转录组和基因组数据阻碍了在分子水平上对蜘蛛毒素的进一步了解。在本研究中，我们将下一代测序和传统的DNA测序相结合，构建了蜘蛛毒腺转录组，通过组装、翻译、过滤、量化和注释，分别从34,334个从头序列和1,024个cDNA序列中鉴定出9,666个和480个高可信蛋白质。对这些蛋白质的广泛功能分析表明，参与RNA转运和剪接体、蛋白质翻译、加工和转运的mRNAs在毒腺中高度丰富，这与毒腺的特殊功能即毒素的产生是一致的。此外，我们还鉴定了12个家族的146个类毒素蛋白，其中包括6个新家族，其中α-LTx-Lt1a家族2首次被鉴定为α-LTx-Lt1a家族的一个亚家族。这些毒素根据其生物活性被分为五类，它们以一种协调的方式发挥作用。在毒腺细胞中表达的离子通道很少，这可能是一种保护自身免受毒素攻击的机制。本研究提供了蜘蛛腺体转录组的图谱，加深了我们对蜘蛛毒素组和毒素产生的蛋白质表达量协调机制的理解。
Latrodectus tredecimguttatus, commonly known as black widow spider, is well known for its dangerous bite. Although its venom has been characterized extensively, some fundamental questions about its molecular composition remain unanswered. The limited transcriptome and genome data available prevent further understanding of spider venom at the molecular level. In the present study, we combined next-generation sequencing and conventional DNA sequencing to construct a venom gland transcriptome of the spider L. tredecimguttatus, which resulted in the identification of 9,666 and 480 high-confidence proteins among 34,334 de novo sequences and 1,024 cDNA sequences, respectively, by assembly, translation, filtering, quantification and annotation. Extensive functional analyses of these proteins indicated that mRNAs involved in RNA transport and spliceosome, protein translation, processing and transport were highly enriched in the venom gland, which is consistent with the specific function of venom glands, namely the production of toxins. Furthermore, we identified 146 toxin-like proteins forming 12 families, including 6 new families in this spider in which α-LTX-Lt1a family2 is firstly identified as a subfamily of α-LTX-Lt1a family. The toxins were classified according to their bioactivities into five categories that functioned in a coordinate way. Few ion channels were expressed in venom gland cells, suggesting a possible mechanism of protection from the attack of their own toxins. The present study provides a gland transcriptome profile and extends our understanding of the toxinome of spiders and coordination mechanism for toxin production in protein expression quantity.
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DOI：
10.1111/j.1432-1033.1993.tb17741.x
发表时间：
1993-04-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
KIYATKIN, N;DULUBOVA, I;GRISHIN, E
通讯作者：
GRISHIN, E
影响因子：
3.5
作者：
KIYATKIN, NI;DULUBOVA, IE;GRISHIN, EV
通讯作者：
GRISHIN, EV
影响因子：
4.7
作者：
Kubista, Helmut;Mafra, Roberta A.;Kuhn-Nentwig, Lucia
通讯作者：
Kuhn-Nentwig, Lucia
影响因子：
4.8
作者：
Dulubova, IE;Krasnoperov, VG;Usherwood, PNR
通讯作者：
Usherwood, PNR
DOI：
10.1073/pnas.1003379107
发表时间：
2010-07-06
影响因子：
11.1
作者：
Kirkness, Ewen F.;Haas, Brian J.;Pittendrigh, Barry R.
通讯作者：
Pittendrigh, Barry R.