Characterization of miRNAs associated with Botrytis cinerea infection of tomato leaves.
Characterization of miRNAs associated with Botrytis cinerea infection of tomato leaves.
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番茄叶片灰霉病感染相关 miRNA 的表征
DOI：
10.1186/s12870-014-0410-4
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发表时间：
2015-01-16
影响因子：
5.3
通讯作者：
Wu F
中科院分区：
文献类型：
--
作者：
Jin W;Wu F
BackgroundBotrytis cinereaPers。赤霉病菌是室内长期栽培番茄茎腐病的重要病原菌。MicroRNAs （miRNAs）已被报道为与多种应激反应和b。番茄灰霉病。然而，mirna在抗b抗体中的作用。cinerearemains不清楚。结果番茄中miRNA表达谱对b。采用高通量测序技术对其进行研究。共鉴定出143个已知mirna和7个新的mirna，并在mock-和b中检测到相应的表达。cinerea-inoculated树叶。其中，1个新的和57个已知的mirna在b中差异表达。其中8个经定量反转录PCR （qRT-PCR）进一步证实。此外，这8个差异表达的mirna中有5个可以通过CleaveLand管道和psRNAtarget程序击中10个编码序列（CDSs）。此外，qRT-PCR结果显示，4个靶点与其对应的mirna （miR319、miR394和miRn1）呈负相关。结论sRNA高通量测序结果显示，mirna的上调可能与番茄对b的反应机制有关。cinereastress。b .表达谱分析。番茄叶片响应mirna及其靶标强烈提示miR319、miR394和miRn1可能参与番茄叶片对b的响应。cinereainfection。
BackgroundBotrytis cinereaPers. Fr. is an important pathogen causing stem rot in tomatoes grown indoors for extended periods. MicroRNAs (miRNAs) have been reported as gene expression regulators related to several stress responses andB. cinereainfection in tomato. However, the function of miRNAs in the resistance toB. cinerearemains unclear.ResultsThe miRNA expression patterns in tomato in response toB. cinereastress were investigated by high-throughput sequencing. In total, 143 known miRNAs and seven novel miRNAs were identified and their corresponding expression was detected in mock- andB. cinerea-inoculated leaves. Among those, one novel and 57 known miRNAs were differentially expressed inB. cinerea-infected leaves, and 8 of these were further confirmed by quantitative reverse-transcription PCR (qRT-PCR). Moreover, five of these eight differentially expressed miRNAs could hit 10 coding sequences (CDSs) via CleaveLand pipeline and psRNAtarget program. In addition, qRT-PCR revealed that four targets were negatively correlated with their corresponding miRNAs (miR319, miR394, and miRn1).ConclusionResults of sRNA high-throughput sequencing revealed that the upregulation of miRNAs may be implicated in the mechanism by which tomato respond toB. cinereastress. Analysis of the expression profiles ofB. cinerea-responsive miRNAs and their targets strongly suggested that miR319, miR394, and miRn1 may be involved in the tomato leaves’ response toB. cinereainfection.
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影响因子：
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作者：
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通讯作者：
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