Synergistic roles of Wnt modulators R-spondin2 and R-spondin3 in craniofacial morphogenesis and dental development.
Synergistic roles of Wnt modulators R-spondin2 and R-spondin3 in craniofacial morphogenesis and dental development.
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Wnt调节剂R-spondin 2和R-spondin 3在颅面形态发生和牙齿发育中的协同作用
DOI：
10.1038/s41598-021-85415-y
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发表时间：
2021-03-12
影响因子：
4.6
通讯作者：
Liao EC
中科院分区：
文献类型：
--
作者：
Alhazmi N;Carroll SH;Kawasaki K;Woronowicz KC;Hallett SA;Macias Trevino C;Li EB;Baron R;Gori F;Yelick PC;Harris MP;Liao EC
Wnt信号传导在颅面模式以及牙齿和骨骼发育中起着关键作用。Rspo2和Rspo3是Wnt信号传导的关键调节因子。然而，它们在颅面发育和牙发生中的协调功能和相对需求却知之甚少。我们发现，在斑马鱼中，rspo 2和rspo 3都在胚胎颅面骨骼的骨祖细胞中表达。这与小鼠发育相反，在小鼠发育中，Rspo3在骨祖细胞中表达，而未观察到Rspo2表达。在斑马鱼中，rspo 2和rspo 3广泛表达于牙髓、成牙本质细胞和上皮隐窝中。然而，在小鼠的磨牙发育中，Rspo3主要表达于牙囊和牙槽间充质中，而Rspo2表达仅限于牙胚。虽然小鼠中的Rspo3消融是胚胎致死的，但斑马鱼rspo3-/-突变体是可行的，并且Meckel软骨喙长度适度减少。然而，rspo3和rspo2的复合破坏揭示了这些基因在软骨形态发生、鳍发育和咽齿发育中的协同作用。成年rspo 3 −/−斑马鱼突变体表现出畸形的颅骨骼和平均牙齿数量减少。这项研究强调了Rspo2和Rspo3在斑马鱼和小鼠的牙颅形态发生中的差异功能。
Wnt signaling plays a critical role in craniofacial patterning, as well as tooth and bone development. Rspo2 and Rspo3 are key regulators of Wnt signaling. However, their coordinated function and relative requirement in craniofacial development and odontogensis are poorly understood. We showed that in zebrafish rspo2 and rspo3 are both expressed in osteoprogenitors in the embryonic craniofacial skeleton. This is in contrast to mouse development, where Rspo3 is expressed in osteoprogenitors while Rspo2 expression is not observed. In zebrafish, rspo2 and rspo3 are broadly expressed in the pulp, odontoblasts and epithelial crypts. However, in the developing molars of the mouse, Rspo3 is largely expressed in the dental follicle and alveolar mesenchyme while Rspo2 expression is restricted to the tooth germ. While Rspo3 ablation in the mouse is embryonic lethal, zebrafish rspo3-/- mutants are viable with modest decrease in Meckel’s cartilage rostral length. However, compound disruption of rspo3 and rspo2 revealed synergistic roles of these genes in cartilage morphogenesis, fin development, and pharyngeal tooth development. Adult rspo3−/− zebrafish mutants exhibit a dysmorphic cranial skeleton and decreased average tooth number. This study highlights the differential functions of Rspo2 and Rspo3 in dentocranial morphogenesis in zebrafish and in mouse.
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影响因子：
2.7
作者：
Ling IT;Rochard L;Liao EC
通讯作者：
Liao EC
DOI：
10.1083/jcb.201207142
发表时间：
2013-02-18
期刊：
The Journal of cell biology
影响因子：
--
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通讯作者：
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影响因子：
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通讯作者：
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作者：
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影响因子：
64.8
作者：
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