Structural Abnormalities of Spermatozoa in Triploid Gynogenetic Crucian Carp (Carassius auratus).
Structural Abnormalities of Spermatozoa in Triploid Gynogenetic Crucian Carp (Carassius auratus).
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三倍体雌核发育鲫鱼精子结构异常
DOI：
10.3389/fgene.2021.783014
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发表时间：
2021
影响因子：
3.7
通讯作者：
Liu S
中科院分区：
文献类型：
--
作者：
He W;Sun Y;Qiang J;Luo X;Zhang H;Yang C;Luo K;Zhao R;Qin Q;Zhang C;Liu S
雌核发育三倍体鲫（Carassius auratus）精子发生过程具有正常的遗传背景。然而，这些精子的遗传物质一般不完全遗传雌核发育后代。了解雌核发育的内在机制有助于雌核发育鱼类育种策略的制定。本研究对二倍体红鲫和3nDTCC精子的超微结构进行了系统的研究，以揭示它们在细胞学结构上的差异。此外，还进行了不同倍性精子对3nDTCC（E100）的人工繁育试验，以验证3nDTCC精子遗传物质对雌核发育后代的贡献。此外，中心粒相关基因（即，cep57、cetn1、rootaoke和nek 2）的表达，以阐明3nDTCC中精子包装过程的分子表达特征。这些结果揭示了3nDTCC中精子的适应性特征，包括松散的中段结构、异常的头部结构以及中心粒相关基因的异常表达，这些特征可能影响精子的运动能力，使其不能正常参与雌核发育后代的遗传组成。
The spermatozoa of triploid gynogenetic crucian carp (Carassius auratus) (3nDTCC) possess a spermatogenesis process with a normal genetic background. However, the genetic materials of these spermatozoa do not completely inherit gynogenetic progeny in general. Understanding the intrinsic mechanism may be helpful for developing breeding strategies of gynogenetic fishes. In this study, the spermatozoa ultrastructure was systematically studied in diploid red crucian carp and 3nDTCC to demonstrate their cytological structural differences. In addition, the artificial breeding tests of 3nDTCC(♀) with different ploidy spermatozoa were performed to verify the contributions of genetic materials from 3nDTCC spermatozoa to the gynogenesis progeny. Furthermore, the mRNA expression of centriole-related genes (i.e., cep57, cetn1, rootletin, and nek2) involved in spermatozoa packaging was also determined by quantitative real-time PCR (qPCR) to illustrate the molecular expression characteristics of the spermatozoa packaging process in 3nDTCC. The results reveal the adaptive features of spermatozoa in 3nDTCC, including the loose midpiece structure, abnormal head structure, and abnormal expression of centriole-related genes, which may influence the motility of spermatozoa and make it not involved normally in the genetic composition of the gynogenesis offspring.
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影响因子：
2.9
作者：
Xiao J;Zou T;Chen Y;Chen L;Liu S;Tao M;Zhang C;Zhao R;Zhou Y;Long Y;You C;Yan J;Liu Y
通讯作者：
Liu Y
多倍体银鲫克隆多样性和双重繁殖模式的遗传基础及育种应用
影响因子：
9.1
作者：
Gui JianFang;Zhou Li
通讯作者：
Zhou Li
额外微染色体在多倍体银鲫中起雄性决定作用
影响因子：
3.3
作者：
Li, Xi-Yin;Zhang, Qi-Ya;Gui, Jian-Fang
通讯作者：
Gui, Jian-Fang
影响因子：
4.1
作者：
Avidor-Reiss T;Carr A;Fishman EL
通讯作者：
Fishman EL
影响因子：
2.9
作者：
Psenicka, Martin;Rodina, Marek;Linhart, Otomar
通讯作者：
Linhart, Otomar