Coexistence of diploid, triploid and tetraploid crucian carp (Carassius auratus) in natural waters.
Coexistence of diploid, triploid and tetraploid crucian carp (Carassius auratus) in natural waters.
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DOI：
10.1186/1471-2156-12-20
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发表时间：
2011-01-29
期刊：
影响因子：
2.9
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Xiao J;Zou T;Chen Y;Chen L;Liu S;Tao M;Zhang C;Zhao R;Zhou Y;Long Y;You C;Yan J;Liu Y
鲫鱼(简称CC)是鲤科鲫属的一种。它是我国水产养殖中最重要的淡水物种之一，在湖南省洞庭湖水系中尤为丰富。CC过去被认为是所有的二倍体形式。而洞庭湖水系的二倍体(简称2nCC)、三倍体(简称3nCC)和四倍体(简称4nCC)种群的共存是我们最近的研究首次发现的。检测了不同倍性CC的倍性水平，比较了不同倍性CC的生物学特性。在生殖方式上，2nCC为两性生殖，4nCC通过雌核发育产生全雌性后代。然而，3nCC以两种不同的方式产生后代。3nCC产生与3nCC精子受精的双性三倍体后代，而当其卵子被异源精子激活时，它通过雌核发育产生全雌性三倍体后代。对三种不同倍性鱼类的线粒体DNA进行了测序和分析，结果表明没有显著差异。有趣的是，仅在3nCC中发现了微染色体，这被认为是杂交的结果。3nCC和4nCC中均有SOX基因的同源DNA片段，而2nCC中没有。基于Sox4基因的系统发育分析表明，3nCC和4nCC与2nCC是一个独立的类群。综上所述，这是中国天然水域中三种不同倍性鲫鱼共存的首次报道。证明了不同倍性CC的共存是可复制的。我们进一步推测3nCC和4nCC是杂交产生的异源多倍体。本研究获得的不同倍性CC群体对脊椎动物多倍化和进化的研究具有重要意义。
Crucian carp (abbreviated CC) belongs to the genus of Carassius within the family of Cyprinidae. It has been one of the most important freshwater species for Chinese aquaculture and is especially abundant in the Dongting water system of Hunan province. CC used to be considered as all diploid forms. However, coexistence of diploid (abbreviated 2nCC), triploid (abbreviated 3nCC) and tetraploid crucian carp (abbreviated 4nCC) population of the Dongting water system was first found by our recently researches. We examined the ploidy level and compared biological characteristics in different ploidy CC. In reproductive mode, 2nCC was bisexual generative and 4nCC generated all-female offspring by gynogenesis. However, 3nCC generated progenies in two different ways. 3nCC produced bisexual triploid offspring fertilized with 3nCC spermatozoa, while it produced all-female triploid offspring by gynogenesis when its ova were activated by heterogenous spermatozoa. The complete mitochondrial DNA of three different ploidy fishes was sequenced and analyzed, suggesting no significant differences. Interestingly, microchromosomes were found only in 3nCC, which were concluded to be the result of hybridization. Allogenetic DNA fragments of Sox genes were obtained in 3nCC and 4nCC, which were absent in 2nCC. Phylogenetics analysis based on Sox4 gene indicated 3nCC and 4nCC formed a separate group from 2nCC. In summary, this is the first report of the co-existence of three types of different ploidy crucian carps in natural waters in China. It was proved that the coexistence of different ploidy CC was reproductively maintained. We further hypothesized that 3nCC and 4nCC were allopolyploids that resulted from hybridization. The different ploidy CC population we obtained in this study possesses great significance for the study of polyploidization and the evolution of vertebrates.
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影响因子：
56.9
作者：
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通讯作者：
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作者：
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影响因子：
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作者：
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通讯作者：
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