Comparison of larval and adult Drosophila astrocytes reveals stage-specific gene expression profiles.
Comparison of larval and adult Drosophila astrocytes reveals stage-specific gene expression profiles.
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DOI：
10.1534/g3.114.016162
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发表时间：
2015-02-04
期刊：
影响因子：
--
通讯作者：
Jackson FR
中科院分区：
文献类型：
--
作者：
Huang Y;Ng FS;Jackson FR
成人星形胶质细胞的神经胶质细胞的分析揭示了一个显着的异质性方面的形态，分子特征，和生理。神经胶质生物学中的一个关键问题是这种异质性如何在大脑发育过程中产生。这个问题的一种方法是确定在发育过程中具有差异星形胶质细胞表达的基因;在神经发育后期表达的某些基因可能有助于星形胶质细胞分化。我们利用果蝇模型和翻译核糖体亲和纯化（TRAP）-RNA-seq方法首次推导出果蝇幼虫星形胶质细胞样细胞（以下简称星形胶质细胞）的全基因组表达谱。这些研究确定了数百个幼虫星形胶质细胞富集的基因，这些基因编码对代谢、能量产生和蛋白质合成重要的蛋白质，这与星形胶质细胞在神经元代谢支持中的已知作用一致。幼虫的轮廓与观察到的成年人的比较已经确定了基因与星形胶质细胞富集表达特定的成年。这些包括对代谢和能量产生、翻译、染色质修饰、蛋白质糖基化、神经肽信号传导、免疫应答、囊泡介导的运输或分泌以及行为调节重要的基因。在这些功能类别中，基于基因本体论分析，对染色质修饰和囊泡介导的运输或分泌重要的基因的表达在成体星形胶质细胞中过度表达。具有选择性成人富集的某些基因可能介导特定于该阶段的功能，或者可能对成人星形胶质细胞的分化或维持很重要，后者可能有助于群体异质性。
The analysis of adult astrocyte glial cells has revealed a remarkable heterogeneity with regard to morphology, molecular signature, and physiology. A key question in glial biology is how such heterogeneity arises during brain development. One approach to this question is to identify genes with differential astrocyte expression during development; certain genes expressed later in neural development may contribute to astrocyte differentiation. We have utilized the Drosophila model and Translating Ribosome Affinity Purification (TRAP)-RNA-seq methods to derive the genome-wide expression profile of Drosophila larval astrocyte-like cells (hereafter referred to as astrocytes) for the first time. These studies identified hundreds of larval astrocyte-enriched genes that encode proteins important for metabolism, energy production, and protein synthesis, consistent with the known role of astrocytes in the metabolic support of neurons. Comparison of the larval profile with that observed for adults has identified genes with astrocyte-enriched expression specific to adulthood. These include genes important for metabolism and energy production, translation, chromatin modification, protein glycosylation, neuropeptide signaling, immune responses, vesicle-mediated trafficking or secretion, and the regulation of behavior. Among these functional classes, the expression of genes important for chromatin modification and vesicle-mediated trafficking or secretion is overrepresented in adult astrocytes based on Gene Ontology analysis. Certain genes with selective adult enrichment may mediate functions specific to this stage or may be important for the differentiation or maintenance of adult astrocytes, with the latter perhaps contributing to population heterogeneity.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
5.3
作者：
Awasaki, Takeshi;Lai, Sen-Lin;Lee, Tzumin
通讯作者：
Lee, Tzumin
影响因子：
6.2
作者：
Brown, Angus M.;Ransom, Bruce R.
通讯作者：
Ransom, Bruce R.
DOI：
10.1523/jneurosci.5951-08.2009
发表时间：
2009-04-15
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Doherty J;Logan MA;Taşdemir OE;Freeman MR
通讯作者：
Freeman MR
影响因子：
16.2
作者：
Halassa, Michael M.;Florian, Cedrick;Fellin, Tommaso;Munoz, James R.;Lee, So-Young;Abel, Ted;Haydon, Philip G.;Frank, Marcos G.
通讯作者：
Frank, Marcos G.