Evaluation of Antifungal Activity and Mechanism of Action of Citral against Candida albicans.
Evaluation of Antifungal Activity and Mechanism of Action of Citral against Candida albicans.
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DOI：
10.1155/2014/378280
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Lima Ede O
中科院分区：
文献类型：
--
作者：
Leite MC;Bezerra AP;de Sousa JP;Guerra FQ;Lima Ede O
白色念珠菌是一种寄生在人体内的酵母菌，可引起机会性或致病性感染。目标。目的：探讨柠檬醛对白色念珠菌的抗真菌活性。方法。采用肉汤微量稀释法测定了菌体的最小抑菌浓度（MIC）和最小杀真菌浓度（MFC）。我们还研究了柠檬醛对细胞壁（0.8 M山梨醇）、细胞膜（柠檬醛与麦角甾醇结合）的可能作用、时间杀伤曲线以及酵母形态的生物活性。结果。柠檬醛的MIC和MFC分别为64µg/mL和256µg/mL。参与细胞壁和麦角甾醇结合被排除为可能的作用机制。在形态干扰实验中，观察到该产物对假菌丝和衣孢子的形成有抑制作用。柠檬醛的MIC和MFC只需暴露4小时即可有效杀死99.9%的接种物。结论。柠檬醛对白色念珠菌具有体外抑菌作用。柠檬醛的作用机制不涉及细胞壁或麦角甾醇，在将来作为新型抗真菌药物的成分使用之前，需要进一步的研究来完整地描述其作用。
Candida albicans is a yeast that commensally inhabits the human body and can cause opportunistic or pathogenic infections. Objective. To investigate the antifungal activity of citral against C. albicans. Methodology. The minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were determined by the broth microdilution techniques. We also investigated possible citral action on cell walls (0.8 M sorbitol), cell membranes (citral to ergosterol binding), the time-kill curve, and biological activity on the yeast's morphology. Results. The MIC and MFC of citral were, respectively, 64 µg/mL and 256 µg/mL. Involvement with the cell wall and ergosterol binding were excluded as possible mechanisms of action. In the morphological interference assay, it was observed that the product inhibited pseudohyphae and chlamydoconidia formation. The MIC and the MFC of citral required only 4 hours of exposure to effectively kill 99.9% of the inoculum. Conclusion. Citral showed in vitro antifungal potential against strains of C. albicans. Citral's mechanism of action does not involve the cell wall or ergosterol, and further study is needed to completely describe its effects before being used in the future as a component of new antifungals.
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DOI：
10.1016/j.ijfoodmicro.2009.10.030
发表时间：
2010-01-01
影响因子：
5.4
作者：
Belletti, Nicoletta;Kamdem, Sylvain Sado;Gardini, Fausto
通讯作者：
Gardini, Fausto
影响因子：
9.4
作者：
Espinel-Ingroff, A;Chaturvedi, V;Rinaldi, MG
通讯作者：
Rinaldi, MG
影响因子：
5.1
作者：
Escalante, Andrea;Gattuso, Martha;Zacchino, Susana
通讯作者：
Zacchino, Susana
影响因子：
2
作者：
Alves, Livia Araujo;Freires, Irlan de Almeida;de Castro, Ricardo Dias
通讯作者：
de Castro, Ricardo Dias
影响因子：
2.8
作者：
de Billerbeck, VG;Roques, CG;Dargent, R
通讯作者：
Dargent, R