Flow-through immunosensors using antibody-immobilized polymer monoliths.
Flow-through immunosensors using antibody-immobilized polymer monoliths.
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DOI：
10.1016/j.bios.2010.06.007
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发表时间：
2010-09-15
影响因子：
12.6
通讯作者：
DeVoe DL
中科院分区：
文献类型：
--
作者：
Liu J;Chen CF;Chang CW;DeVoe DL
研究了基于光聚合表面反应性聚合物单体的高灵敏度、快速流动免疫传感器。以甲基丙烯酸缩水甘油酯和乙氧基化三甲基丙烷三丙烯酸酯前体为原料，在二氧化硅毛细管内合成了多孔单体，提供了具有高表面积的弯曲孔结构，用于固定抗体或其他生物传感配体。单体的独特形态确保了高效的质量运输和溶剂化分析物分子之间的相互作用，以及共价固定在单体表面的抗体，从而实现快速的免疫识别。这种方法的有效性通过使用抗IgG作为单片结合捕获抗体和荧光素标记的IgG作为抗原的直接免疫分析模型得到了证明。原位抗原检测在0.1 ~ 50 ng/mL的浓度范围内表现出线性响应，检测时间为5 min，而通过整体元件可控注射1 μL体积的抗原，质量检测限为100 pg （~700 amol）。这些结果表明，多孔整体支架是一种灵活而有前途的材料，可用于制造快速灵敏的免疫传感器，适合集成到毛细管或微流体装置中。
High-sensitivity and rapid flow-through immunosensors based on photopolymerized surface-reactive polymer monoliths are investigated. The porous monoliths were synthesized within silica capillaries from glycidyl methacrylate and ethoxylated trimethylolpropane triacrylate precursors, providing a tortuous pore structure with high surface area for the immobilization of antibodies or other biosensing ligands. The unique morphology of the monolith ensures efficient mass transport and interactions between solvated analyte molecules and covalently immobilize antibodies anchored to the monolith surface, resulting in rapid immunorecognition. The efficacy of this approach is demonstrated through a direct immunoassay model using anti-IgG as a monolith-bound capture antibody and fluorescein-labeled IgG as an antigen. In situ antigen measurements exhibited a linear response over a concentration range between 0.1 - 50 ng/mL with 5 min assay times, while controllable injection of 1 μL volumes of antigen through the monolith elements yielded a mass detection limit of 100 pg (~700 amol). These results suggest that porous monolith supports represent a flexible and promising material for the fabrication of rapid and sensitive immunosensors suitable for integration into capillary or microfluidic devices.
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影响因子：
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