A reversibly gated protein-transporting membrane channel made of DNA.
A reversibly gated protein-transporting membrane channel made of DNA.
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DOI：
10.1038/s41467-022-28522-2
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发表时间：
2022-04-28
影响因子：
16.6
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中科院分区：
文献类型：
--
作者：
生物分子在脂双层膜中的受控传输在生物过程中具有深远的意义。在细胞中，货物交换是由专用通道介导的，这些通道响应于触发器，经历纳米机械变化以可逆地打开，从而调节货物通量。用简单但可编程的化学方法复制这些过程具有根本的科学意义。在运输控制和货物方面超越自然能力的人工系统对生物技术应用也有相当大的兴趣，但建造起来具有挑战性。在这里，我们描述了一种合成通道，允许精确定时，刺激控制的折叠和功能蛋白质跨双层膜的运输。该通道通过DNA纳米技术设计原理制成，具有416 nm 2的开口横截面和纳米机械盖，该盖可以通过锁和钥匙机制可控地关闭和重新打开。我们设想功能性DNA装置可用于高灵敏度的生物传感、蛋白质的药物递送以及人工细胞网络的创建。控制分子跨生物膜运输的人工系统可用于生物传感或药物递送。在这里，作者组装了一个DNA通道，能够精确定时，刺激控制功能蛋白质跨双层膜的运输。
Controlled transport of biomolecules across lipid bilayer membranes is of profound significance in biological processes. In cells, cargo exchange is mediated by dedicated channels that respond to triggers, undergo a nanomechanical change to reversibly open, and thus regulate cargo flux. Replicating these processes with simple yet programmable chemical means is of fundamental scientific interest. Artificial systems that go beyond nature’s remit in transport control and cargo are also of considerable interest for biotechnological applications but challenging to build. Here, we describe a synthetic channel that allows precisely timed, stimulus-controlled transport of folded and functional proteins across bilayer membranes. The channel is made via DNA nanotechnology design principles and features a 416 nm2 opening cross-section and a nanomechanical lid which can be controllably closed and re-opened via a lock-and-key mechanism. We envision that the functional DNA device may be used in highly sensitive biosensing, drug delivery of proteins, and the creation of artificial cell networks. Artificial systems to control the transport of molecules across biomembranes can be useful for biosensing or drug delivery. Here, the authors assemble a DNA channel enabling the precisely timed, stimulus-controlled transport of functional proteins across bilayer membranes.
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影响因子：
10.8
作者：
Göpfrich K;Li CY;Mames I;Bhamidimarri SP;Ricci M;Yoo J;Mames A;Ohmann A;Winterhalter M;Stulz E;Aksimentiev A;Keyser UF
通讯作者：
Keyser UF
影响因子：
14.9
作者：
Douglas SM;Marblestone AH;Teerapittayanon S;Vazquez A;Church GM;Shih WM
通讯作者：
Shih WM
影响因子：
17.1
作者：
Arulkumaran, Nishkantha;Lanphere, Conor;Howorka, Stefan
通讯作者：
Howorka, Stefan
影响因子：
25
作者：
Banghart, M;Borges, K;Kramer, RH
通讯作者：
Kramer, RH
影响因子：
38.3
作者：
Burns, Jonathan R.;Seifert, Astrid;Howorka, Stefan
通讯作者：
Howorka, Stefan