Non-Targeted Metabolomics Reveals Patterns of Metabolic Changes during Poplar Seed Germination
Non-Targeted Metabolomics Reveals Patterns of Metabolic Changes during Poplar Seed Germination
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非靶向代谢组学揭示杨树种子萌发过程中的代谢变化模式
作者：
Chunpu Qu;Jinyuan Chen;Lina Cao;Xiangjin Teng;Jinbo Li;Chengjun Yang;Xiuli Zhang;Yuhong Zhang;Guanjun Liu;Zhiru Xu
研究重点：本研究首次利用代谢组学技术对杨树种子萌发进行了研究。研究结果为揭示木本植物种子萌发过程中代谢物水平的变化及其机制奠定了基础。背景与目的：白杨是一种模式木本植物。由于杨树易于无性繁殖，杨树种子萌发的分子机制尚未得到很好的研究。但长期无性繁殖导致杨树幼苗抗性弱、易退化、生长势下降。材料与方法：利用非靶向代谢组学技术分析了杨树种子萌发过程中代谢物含量的变化趋势。结果：我们发现，随着种子萌发的进展，差异代谢产物的数量增加。差异代谢产物的代谢途径分析表明，半乳糖代谢和丙氨酸，天冬氨酸和谷氨酸代谢显着丰富在所有萌发期。基于MapMan的差异代谢产物代谢途径的可视化分析表明，谷氨酰胺，谷氨酸，苯丙氨酸，精氨酸和天冬酰胺含量增加发芽时间，与大多数代谢产物相关的葡萄糖代谢遵循类似的趋势。与三羧酸循环相关的代谢产物含量呈波动性变化。结论：本研究揭示了杨树种子萌发过程中初级代谢物含量的主要变化规律，为阐明杨树种子萌发的分子机理奠定了基础。
Research Highlights: This study was the first to use metabolomics techniques to investigate seed germination in poplar, a model woody plant. Our results lay a foundation for uncovering changes in metabolite levels during woody plant seed germination and for understanding the underlying mechanism. Background and Objectives: Poplar is a model woody plant. Because poplar can be easily propagated asexually, the molecular mechanism of poplar seed germination has not been well studied. However, long-term asexual reproduction of poplar leads to seedlings with weak resistance, high vulnerability to degradation, and reduced growth potential. Materials and Methods: The non-targeted metabolomics technique was used to analyze changing trends in metabolite contents during the poplar seed germination process. Results: We found that the number of differential metabolites increased as seed germination progressed. Metabolic pathway analysis of differential metabolites revealed that galactose metabolism and alanine, aspartate, and glutamate metabolism were significantly enriched during all germination periods. MapMan-based visual analysis of metabolic pathways of differential metabolites indicated that glutamine, glutamic acid, phenylalanine, arginine, and asparagine contents increased with germination time, with most metabolites related to glucose metabolism following similar trends. Contents of most metabolites related to the tricarboxylic acid cycle exhibited a fluctuating pattern. Conclusions: This study has revealed the major changes taking place in primary metabolite contents during poplar seed germination and has laid the foundation for elucidation of the molecular mechanism of poplar seed germination.
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