Crosslinked elastic fibers are necessary for low energy loss in the ascending aorta.
Crosslinked elastic fibers are necessary for low energy loss in the ascending aorta.
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DOI：
10.1016/j.jbiomech.2017.07.011
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发表时间：
2017-08-16
影响因子：
2.4
通讯作者：
Wagenseil JE
中科院分区：
文献类型：
--
作者：
Kim J;Staiculescu MC;Cocciolone AJ;Yanagisawa H;Mecham RP;Wagenseil JE
在大动脉中，据信弹性蛋白提供在低压下拉伸的阻力，而胶原蛋白提供在高压下拉伸的阻力。人们还认为，弹性蛋白是负责低能量损失观察到的循环负荷。这些原则通过实验得到支持，这些实验通过蛋白酶消化、血管重塑、正常生长或不同动脉类型来改变组分量。基因工程提供了机会，重新审视这些原则，通过表达的特定壁组件的损失。我们使用缺乏弹性蛋白（Eln−/−）或两种关键蛋白质（赖氨酰氧化酶，Lox−/−或fibulin-4，Fbln 4 −/−）的新生小鼠，这些蛋白质是机械功能弹性纤维组装所必需的，以研究弹性纤维对大动脉力学的贡献。我们测定了Eln−/−、Lox−/−和Fbln 4 −/−升主动脉及其各自对照的组分含量和组织，并量化了其非线性和粘弹性力学行为。我们证实，缺乏弹性蛋白，fibulin-4，或赖氨酰氧化酶导致缺乏或高度破碎的弹性纤维在主动脉壁和交联弹性蛋白量减少56 - 97%。我们发现，只有Eln−/−主动脉在低压下的拉伸阻力降低，证实了弹性蛋白在主动脉壁非线性力学行为中的作用。在Eln−/−、Lox−/−和Fbln 4 −/−主动脉中，循环加载和卸载的耗散能量增加了53 - 387%，这表明不仅弹性蛋白，而且适当组装和交联的弹性纤维对于主动脉中的低能量损失是必要的。
In the large arteries, it is believed that elastin provides the resistance to stretch at low pressure, while collagen provides the resistance to stretch at high pressure. It is also thought that elastin is responsible for the low energy loss observed with cyclic loading. These tenets are supported through experiments that alter component amounts through protease digestion, vessel remodeling, normal growth, or in different artery types. Genetic engineering provides the opportunity to revisit these tenets through the loss of expression of specific wall components. We used newborn mice lacking elastin (Eln−/−) or two key proteins (lysyl oxidase, Lox−/−, or fibulin-4, Fbln4−/−) that are necessary for the assembly of mechanically-functional elastic fibers to investigate the contributions of elastic fibers to large artery mechanics. We determined component content and organization and quantified the nonlinear and viscoelastic mechanical behavior of Eln−/−, Lox−/−, and Fbln4−/− ascending aorta and their respective controls. We confirmed that the lack of elastin, fibulin-4, or lysyl oxidase leads to absent or highly fragmented elastic fibers in the aortic wall and a 56 – 97% decrease in crosslinked elastin amounts. We found that the resistance to stretch at low pressure is decreased only in Eln−/− aorta, confirming the role of elastin in the nonlinear mechanical behavior of the aortic wall. Dissipated energy with cyclic loading and unloading is increased 53 – 387% in Eln−/−, Lox−/−, and Fbln4−/− aorta, indicating that not only elastin, but properly assembled and crosslinked elastic fibers, are necessary for low energy loss in the aorta.
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影响因子：
2.9
作者：
JAMALL, IS;FINELLI, VN;HEE, SSQ
通讯作者：
HEE, SSQ
DOI：
10.1073/pnas.0908268106
发表时间：
2009-11-10
影响因子：
11.1
作者：
Horiguchi, Masahito;Inoue, Tadashi;Nakamura, Tomoyuki
通讯作者：
Nakamura, Tomoyuki
影响因子：
16.6
作者：
Chaudhuri O;Gu L;Darnell M;Klumpers D;Bencherif SA;Weaver JC;Huebsch N;Mooney DJ
通讯作者：
Mooney DJ
储存持续时间对小鼠颈动脉机械行为的影响。
DOI：
10.1115/1.4004415
发表时间：
2011-07
期刊：
Journal of biomechanical engineering
影响因子：
--
作者：
Amin M;Kunkel AG;Le VP;Wagenseil JE
通讯作者：
Wagenseil JE
影响因子：
20.1
作者：
Huang J;Davis EC;Chapman SL;Budatha M;Marmorstein LY;Word RA;Yanagisawa H
通讯作者：
Yanagisawa H