Effect of storage duration on the mechanical behavior of mouse carotid artery.
Effect of storage duration on the mechanical behavior of mouse carotid artery.
复制标题
储存持续时间对小鼠颈动脉机械行为的影响。
DOI：
10.1115/1.4004415
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发表时间：
2011-07
期刊：
影响因子：
--
通讯作者：
Wagenseil JE
中科院分区：
文献类型：
--
作者：
Amin M;Kunkel AG;Le VP;Wagenseil JE
确定动脉机械特性对于了解心脏所做的工作及其随心血管疾病的变化非常重要。离体测试对于向动脉施加各种负载并获取数据来建模和预测任何负载下的行为是必要的。大多数离体测试是在解剖后 24 小时内进行的，因此组织仍然“活着”。然而，对于大的弹性动脉，被动机械行为主要归因于非常稳定的蛋白质、弹性蛋白和胶原蛋白。如果测试设备出现故障、正在使用中或位于其他设施，则存储容器并推迟测试直到设备可用为止会很有用。本研究的目的是确定储存时间对成年小鼠颈总动脉机械行为的影响。每条动脉在 4°C 生理盐水中保存 1-28 天后进行测试。存储时间对每个压力下的动脉直径或力没有显着影响，但对每个压力下的拉伸比和应力有显着影响。对拉伸比和应力的显着影响是由于从切割的动脉环测量时，卸载尺寸随着存储时间的推移而减小。当卸载尺寸而不是从组织学切片测量时，随着储存时间的推移没有显着变化。我们得出的结论是，组织学切片对卸载尺寸产生了更一致的测量，并且储存长达 28 天的小鼠颈动脉的机械行为没有显着变化。
Determining arterial mechanical properties is important for understanding the work done by the heart and how it changes with cardiovascular disease. Ex vivo tests are necessary to apply various loads to the artery and obtain data to model and predict the behavior under any load. Most ex vivo tests are performed within 24 hours of dissection, so the tissue is still “alive”. For large elastic arteries, however, the passive mechanical behavior is attributed mostly to the very stable proteins, elastin and collagen. If the testing equipment fails, is in use, or is located at another facility, it would be useful to store the vessels and postpone the tests until the equipment is available. The goal of this study is to determine the effects of storage time on the mechanical behavior of the common carotid artery from adult mice. Each artery was tested after storage for 1–28 days in physiologic saline at 4°C. There were no significant effects of storage time on the arterial diameter or force at each pressure, but there were significant effects on the stretch ratio and stress at each pressure. The significant effects on the stretch ratio and stress were due to decreases in the unloaded dimensions with storage time, when measured from cut arterial rings. When the unloaded dimensions were measured instead from histology sections, there were no significant changes with storage time. We conclude that histology sections yield a more consistent measurement of the unloaded dimensions and that there are no significant changes in the mechanical behavior of mouse carotid artery with storage up to 28 days.
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影响因子：
7.3
作者：
McIntyre, CA;Williams, BC;Hadoke, PWF
通讯作者：
Hadoke, PWF
影响因子：
2.2
作者：
Stemper, Brian D.;Yoganandan, Narayan;Pintar, Frank A.
通讯作者：
Pintar, Frank A.
DOI：
10.1152/ajpheart.00046.2005
发表时间：
2005-09-01
影响因子：
4.8
作者：
Wagenseil, JE;Nerurkar, NL;Mecham, RP
通讯作者：
Mecham, RP
影响因子：
2.4
作者：
TAKAMIZAWA, K;HAYASHI, K
通讯作者：
HAYASHI, K
影响因子：
9
作者：
PEIRCE, EC;GROSS, RE;MERRILL, K
通讯作者：
MERRILL, K