Transcriptome and biochemical analyses revealed a detailed proanthocyanidin biosynthesis pathway in brown cotton fiber.
Transcriptome and biochemical analyses revealed a detailed proanthocyanidin biosynthesis pathway in brown cotton fiber.
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转录组和生化分析揭示了棕色棉纤维中详细的原花青素生物合成途径
DOI：
10.1371/journal.pone.0086344
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Pei Y
中科院分区：
文献类型：
--
作者：
Xiao YH;Yan Q;Ding H;Luo M;Hou L;Zhang M;Yao D;Liu HS;Li X;Zhao J;Pei Y
棕棉纤维是彩棉工业的主要原料。此前的研究表明，棉纤维中的棕色色素属于原花青素（PAs）。为了阐明棕色棉纤维中 PA 生物合成途径的细节，通过数字基因表达谱和 qRT-PCR 比较了发育中的棕色和白色纤维的基因表达谱。与白色棉纤维相比，棕色纤维中从苯丙氨酸到 PA 单体（黄烷-3-醇）的所有步骤均显着上调。液相色谱质谱分析表明，棕色纤维中的游离黄烷-3-醇大部分为2, 3-反式（没食子儿茶素和儿茶素），聚合PA的主要单元在B环上被三羟基化。与单体组成一致，棉纤维中类黄酮3'、5'-羟化酶和无色花青素还原酶的转录水平远高于作用于相同底物的竞争酶（分别为二氢黄酮醇4-还原酶和花青素合酶）。综上所述，我们的数据揭示了棕色棉纤维中完全激活的详细 PA 生物合成途径，并证明类黄酮 3', 5'-羟化酶和无色花青素还原酶代表了棉纤维中 PA 生物合成的主要流程。
Brown cotton fiber is the major raw material for colored cotton industry. Previous studies have showed that the brown pigments in cotton fiber belong to proanthocyanidins (PAs). To clarify the details of PA biosynthesis pathway in brown cotton fiber, gene expression profiles in developing brown and white fibers were compared via digital gene expression profiling and qRT-PCR. Compared to white cotton fiber, all steps from phenylalanine to PA monomers (flavan-3-ols) were significantly up-regulated in brown fiber. Liquid chromatography mass spectrometry analyses showed that most of free flavan-3-ols in brown fiber were in 2, 3-trans form (gallocatechin and catechin), and the main units of polymeric PAs were trihydroxylated on B ring. Consistent with monomeric composition, the transcript levels of flavonoid 3′, 5′-hydroxylase and leucoanthocyanidin reductase in cotton fiber were much higher than their competing enzymes acting on the same substrates (dihydroflavonol 4-reductase and anthocyanidin synthase, respectively). Taken together, our data revealed a detailed PA biosynthesis pathway wholly activated in brown cotton fiber, and demonstrated that flavonoid 3′, 5′-hydroxylase and leucoanthocyanidin reductase represented the primary flow of PA biosynthesis in cotton fiber.
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植物中原花青素的生物合成和遗传调控
DOI：
10.3390/molecules13102674
发表时间：
2008-10-28
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
He F;Pan QH;Shi Y;Duan CQ
通讯作者：
Duan CQ
影响因子：
3.7
作者：
Feng H;Tian X;Liu Y;Li Y;Zhang X;Jones BJ;Sun Y;Sun J
通讯作者：
Sun J
影响因子：
4.4
作者：
Chaudhary B;Hovav R;Flagel L;Mittler R;Wendel JF
通讯作者：
Wendel JF
影响因子：
7.4
作者：
Winkel-Shirley, B
通讯作者：
Winkel-Shirley, B
影响因子：
14.9
作者：
Wheeler, DL;Church, DM;Wagner, L
通讯作者：
Wagner, L