Analysis of flavonoids and the flavonoid structural genes in brown fiber of upland cotton.
Analysis of flavonoids and the flavonoid structural genes in brown fiber of upland cotton.
复制标题
DOI：
10.1371/journal.pone.0058820
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Sun J
中科院分区：
文献类型：
--
作者：
Feng H;Tian X;Liu Y;Li Y;Zhang X;Jones BJ;Sun Y;Sun J
由于消费者偏好的变化，具有天然颜色纤维的棉花品种在纺织工业中越来越受欢迎。导致有色纤维发育的分子机制仍然很大程度上是未知的，尽管预计颜色来自黄酮类化合物。首先克隆棉花类黄酮生物合成途径的4个关键基因（GhC4H、GhCHS、GhF3’h和GhF3’5’h），并利用QRT-PCR技术研究其在棉花棕白纤维发育过程中的表达谱。然后，利用高效液相色谱法分析了黄酮生物合成途径中柚皮素、槲皮素、山奈酚和杨梅素4种成分在棕、白纤维不同发育阶段的含量。这些基因对应的4个类黄酮结构基因的预测蛋白与不同植物物种的对应蛋白序列具有很强的相似性。这四个基因的转录水平在发育棕色纤维中明显高于来自近等基因系（NIL）的白色纤维。4种黄酮类化合物（柚皮素、槲皮素、山奈酚和杨梅素）在棕色纤维中的含量显著高于白色纤维，与生物合成基因的表达水平相对应。黄酮类结构基因表达和黄酮类代谢在褐棉纤维色素沉着发育中起重要作用。
As a result of changing consumer preferences, cotton (Gossypium Hirsutum L.) from varieties with naturally colored fibers is becoming increasingly sought after in the textile industry. The molecular mechanisms leading to colored fiber development are still largely unknown, although it is expected that the color is derived from flavanoids. Firstly, four key genes of the flavonoid biosynthetic pathway in cotton (GhC4H, GhCHS, GhF3′H, and GhF3′5′H) were cloned and studied their expression profiles during the development of brown- and white cotton fibers by QRT-PCR. And then, the concentrations of four components of the flavonoid biosynthetic pathway, naringenin, quercetin, kaempferol and myricetin in brown- and white fibers were analyzed at different developmental stages by HPLC. The predicted proteins of the four flavonoid structural genes corresponding to these genes exhibit strong sequence similarity to their counterparts in various plant species. Transcript levels for all four genes were considerably higher in developing brown fibers than in white fibers from a near isogenic line (NIL). The contents of four flavonoids (naringenin, quercetin, kaempferol and myricetin) were significantly higher in brown than in white fibers and corresponding to the biosynthetic gene expression levels. Flavonoid structural gene expression and flavonoid metabolism are important in the development of pigmentation in brown cotton fibers.
登录
查看更多内容
DOI：
10.1111/j.1365-313x.2006.02964.x
发表时间：
2007-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Espley RV;Hellens RP;Putterill J;Stevenson DE;Kutty-Amma S;Allan AC
通讯作者：
Allan AC
影响因子：
7.4
作者：
Bogs, J;Ebadi, A;Robinson, SP
通讯作者：
Robinson, SP
影响因子：
5.6
作者：
Chen HJ;Inbaraj BS;Chen BH
通讯作者：
Chen BH
影响因子：
14.9
作者：
THOMPSON, JD;HIGGINS, DG;GIBSON, TJ
通讯作者：
GIBSON, TJ
红色葡萄藤（Vitis Vinifera L.）的颜色变化：基因组组织，类黄酮3'-羟化酶的表达，类黄酮3'，5'-羟化酶基因以及相关的红色氰化物/蓝色Delphinidin arthocyin atthocyin in Berry sigry simmiken。
影响因子：
4.4
作者：
Castellarin, Simone D;Di Gaspero, Gabriele;Marconi, Raffaella;Nonis, Alberto;Peterlunger, Enrico;Paillard, Sophie;Adam-Blondon, Anne-Francoise;Testolin, Raffaele
通讯作者：
Testolin, Raffaele