Distinct impacts of substrate elasticity and ligand affinity on traction force evolution.
Distinct impacts of substrate elasticity and ligand affinity on traction force evolution.
复制标题
基质弹性和配体亲和力对牵引力演变的独特影响
作者：
Müller C;Pompe T
细胞粘附受细胞环境的机械特性调节。粘附基质的不同参数的影响在细胞反应中是复杂的，导致对潜在机制的问题，如粘附分子水平上的生化信号传导，或基质和细胞的粘弹性。通过对早期细胞粘附过程中牵引力产生的时间分辨分析，我们希望阐明基底力学对粘附过程的贡献，特别是基底弹性和基底表面上粘附配体的分子摩擦的影响。这两个参数独立调整（i）可变交联度的弹性聚丙烯酰胺水凝胶和（ii）薄的聚合物涂层的水凝胶表面控制的亲和力（和相关的底物-配体摩擦）的粘附配体纤连蛋白。我们的分析表明，两个顺序制度的相当大的力的产生，其发生被认为是独立的基板性能。第一个政权的特点是扩展的细胞和随后的力的增加。展开后的细胞进入第二政权与饱和的力量。基质的弹性和粘度，即水凝胶的弹性和配体的亲和力，都被发现影响动力学和绝对水平的牵引力的数量。一个更快的增长和更高的饱和水平的牵引力观察到更高的基板刚度和更高的配体亲和力。结果补充了最近的建模方法的演变力在细胞扩散，并有助于更好地了解粘弹性基板上的细胞粘附的动力学。
Cell adhesion is regulated by the mechanical characteristics of the cell environment. The influences of different parameters of the adhesive substrates are convoluted in the cell response leading to questions on the underlying mechanisms, like biochemical signaling on the level of adhesion molecules, or viscoelastic properties of substrates and cell. By a time-resolved analysis of traction force generation during early cell adhesion, we wanted to elucidate the contributions of substrate mechanics to the adhesion process, in particular the impact of substrate elasticity and the molecular friction of adhesion ligands on the substrate surface. Both parameters were independently adjusted by (i) an elastic polyacrylamide hydrogel of variable crosslinking degree and (ii) a thin polymer coating of the hydrogel surface controlling the affinity (and the correlated substrate–ligand friction) of the adhesion ligand fibronectin. Our analysis showed two sequential regimes of considerable force generation, whose occurrence was found to be independent of substrate properties. The first regime is characterized by spreading of the cell and a succeeding force increase. After spreading cells enter the second regime with saturated forces. Substrate elasticity and viscosity, namely hydrogel elasticity and ligand affinity, were both found to affect the kinetics and absolute levels of traction force quantities. A faster increase and a higher saturation level of traction forces were observed for a higher substrate stiffness and a higher ligand affinity. The results complement recent modeling approaches on the evolution of forces in cell spreading and contribute to a better understanding of the dynamics of cell adhesion on viscoelastic substrates.
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影响因子：
3.4
作者：
Christina Müller;A. Müller;T. Pompe
通讯作者：
Christina Müller;A. Müller;T. Pompe
影响因子：
3.4
作者：
Sabass, Benedikt;Gardel, Margaret L.;Schwarz, Ulrich S.
通讯作者：
Schwarz, Ulrich S.
DOI：
10.1152/ajpcell.00169.2002
发表时间：
2002-10
期刊：
American journal of physiology. Cell physiology
影响因子：
--
作者：
I. Tolic-Nørrelykke;J. Butler;Jianxin Chen;Ning Wang
通讯作者：
I. Tolic-Nørrelykke;J. Butler;Jianxin Chen;Ning Wang
具有分级理化特性的共聚物基底上纤连蛋白层的动态变化。
DOI：
10.1021/la0362627
发表时间：
2004
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
L. Renner;T. Pompe;K. Salchert;C. Werner
通讯作者：
C. Werner
影响因子：
50.3
作者：
Paszek, MJ;Zahir, N;Weaver, VM
通讯作者：
Weaver, VM