Variation in IgE binding potencies of seven Artemisia species depending on content of major allergens.
Variation in IgE binding potencies of seven Artemisia species depending on content of major allergens.
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七种蒿属植物 IgE 结合效力的变化取决于主要过敏原的含量
DOI：
10.1186/s13601-020-00354-7
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发表时间：
2020-11-18
影响因子：
4.4
通讯作者：
van Ree R
中科院分区：
文献类型：
--
作者：
Zhao L;Fu W;Gao B;Liu Y;Wu S;Chen Z;Zhang X;Wang H;Feng Y;Wang X;Wang H;Lan T;Liu M;Wang X;Sun Y;Luo F;Gadermaier G;Ferreira F;Versteeg SA;Akkerdaas JH;Wang D;Valenta R;Vrtala S;Gao Z;van Ree R
背景蒿草花粉过敏在北半球很重要。虽然已记录该属超过 350 个物种，但尚未全面调查不同物种是否可能影响过敏原的诊断和治疗。本研究旨在评价代表性蒿属植物的氨基酸序列变异和主要过敏原含量及其对特异性IgE结合能力的影响。方法以中国6个代表性蒿属植物和欧洲的青蒿为原料，通过纯化的过敏原成分蛋白的转录组、基因测序和质谱分析，确定过敏原氨基酸序列。开发了夹心 ELISA 并应用于不同物种的 Art v 1、Art v 2 和 Art v 3 过敏原定量。采用花粉水提取物和纯化的过敏原成分，通过ELISA和ImmunoCAP技术，对中国五个地区的艾蒿过敏患者血清库和个体血清进行IgE结合评估。结果7个蒿种中Art v 1和Art v 2同源过敏原序列高度保守。 A 中的 Art v 3 型过敏原。 annua和A.与 A 相比，sieversiana 的差异更大。 argyiandA.寻常的。七种提取物中Art v 1组的过敏原含量范围为3.4％至7.1％，Art v 2组的过敏原含量为1.0％至3.6％，Art v 3组的过敏原含量为0.3％至10.5％。大多数艾蒿过敏患者的 IgE 结合效力最高的是 withA。 annuapollen提取物，其次是A.寻常的和A.艾叶，与A.西弗氏体显着降低。不同种属的Natural Art v 1-3同种过敏原在我国蒿类过敏患者中具有几乎相当的IgE结合能力。结论及临床意义不同种属的三组过敏原序列相似性较高，但含量不同。选用黄花蒿和A．中国推荐使用艾草花粉作为诊断和免疫治疗的来源。
BackgroundArtemisiaweed pollen allergy is important in the northern hemisphere. While over 350 species of this genus have been recorded, there has been no full investigation into whether different species may affect the allergen diagnosis and treatment. This study aimed to evaluate the variations in amino acid sequences and the content of major allergens, and how these affect specific IgE binding capacity in representativeArtemisiaspecies.MethodsSix representativeArtemisiaspecies from China andArtemisia vulgarisfrom Europe were used to determine allergen amino acid sequences by transcriptome, gene sequencing and mass spectrometry of the purified allergen component proteins. Sandwich ELISAs were developed and applied for Art v 1, Art v 2 and Art v 3 allergen quantification in different species. Aqueous pollen extracts and purified allergen components were used to assess IgE binding by ELISA and ImmunoCAP with mugwort allergic patient serum pools and individual sera from five areas in China.ResultsThe Art v 1 and Art v 2 homologous allergen sequences in the sevenArtemisiaspecies were highly conserved. Art v 3 type allergens inA. annuaandA. sieversianawere more divergent compared toA. argyiandA. vulgaris. The allergen content of Art v 1 group in the seven extracts ranged from 3.4% to 7.1%, that of Art v 2 from 1.0% to 3.6%, and Art v 3 from 0.3% to 10.5%. The highest IgE binding potency for most Chinese Artemisia allergy patients was withA. annuapollen extract, followed byA. vulgarisandA. argyi, withA. sieversianasignificantly lower. Natural Art v 1-3 isoallergens from different species have almost equivalent IgE binding capacity inArtemisiaallergic patients from China.Conclusion and clinical relevanceThere was high sequence similarity but different content of the three group allergens from differentArtemisiaspecies. Choice ofArtemisia annuaandA. argyipollen source for diagnosis and immunotherapy is recommended in China.
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