Humoral Immune Response of Thai Dogs after Oral Vaccination against Rabies with the SPBN GASGAS Vaccine Strain.
Humoral Immune Response of Thai Dogs after Oral Vaccination against Rabies with the SPBN GASGAS Vaccine Strain.
复制标题
DOI：
10.3390/vaccines8040573
复制
发表时间：
2020-10-01
期刊：
影响因子：
7.8
通讯作者：
Chanachai K
中科院分区：
文献类型：
--
作者：
Leelahapongsathon K;Kasemsuwan S;Pinyopummintr T;Boodde O;Phawaphutayanchai P;Aiyara N;Bobe K;Vos A;Friedrichs V;Müller T;Freuling CM;Chanachai K
应用研究对于突破界限并找到解决狗媒狂犬病这一由来已久的问题至关重要。尽管犬只口服疫苗被认为在大规模的犬类疫苗接种活动中具有巨大的潜力，并可能产生深远的好处，但在努力消除狗传播的狂犬病方面，它可能是所有可用工具中最被忽视的，尤其是因为关于潜在口服狂犬病疫苗候选对象的免疫原性、有效性和安全性的数据有限。在这项研究中，评估了口服高度减毒的第三代狂犬病病毒疫苗株SPBN GASGAS对泰国当地狗的长期免疫原性。将口服狂犬病疫苗直接口服给犬(n=10)或通过提供疫苗肠道诱饵给药(n=15)。然后将体液免疫反应与三组狗进行比较：接受非肠道注射灭活狂犬病疫苗的组(n=10)，提供安慰剂肠道诱饵的组(n=7)，以及对照组(n=4)，观察期为365天。接受口服和注射疫苗的犬在狂犬病病毒(RABV)中和抗体(RFFIT)和结合抗体(ELISA)的大小、动力学和持久性方面没有显著差异。虽然一次性注射灭活狂犬病疫苗的体液免疫反应略高于口服活疫苗，但在所有治疗组的大多数动物中，一年后仍可检测到这两种类型的RABV特异性抗体，且血清阳性率没有差异。狂犬病特异性抗体的特征揭示了参与接种疫苗的狗的免疫反应的两类主要抗体。虽然首先出现的是IgM抗体，但随后的免疫应答主要是以IgG2为主，与所使用的疫苗类型无关。这些结果支持SPBN GASGAS在当地狗体内直接口服和通过诱饵使用都能诱导持续的可检测到的免疫反应的观点。
Applied research is crucial in pushing the boundaries and finding a solution to the age-old problem of dog-mediated rabies. Although oral vaccination of dogs is considered to have great potential in mass dog vaccination campaigns and could have far-reaching benefits, it is perhaps the most ignored of all available tools in efforts to eliminate dog-mediated rabies, not least because of limited data on immunogenicity, efficacy, and safety of potential oral rabies vaccine candidates. In this study, the long-term immunogenicity in local Thai dogs after oral administration of the highly attenuated 3rd generation rabies virus vaccine strain SPBN GASGAS was assessed. The oral rabies vaccine was administered to dogs by either direct oral administration (n = 10) or by offering a vaccine loaded intestine bait (n = 15). The humoral immune response was then compared to three groups of dogs; a group that received a parenteral delivered inactivated rabies vaccine (n = 10), a group offered a placebo intestine bait (n = 7), and a control group (n = 4) for an observation period of 365 days. There was no significant difference in the immune response of dogs that received oral and parenteral vaccine in terms of magnitude, kinetics, and persistence of both rabies virus (RABV) neutralizing (RFFIT) and binding (ELISA) antibodies. Although the single parenteral injection of an inactivated rabies vaccine mounted a slightly higher humoral immune response than the orally delivered live vaccine, RABV specific antibodies of both types were still detectable after one year in most animals for all treatment groups and resulted in no difference in seropositivity. Characterization of rabies specific antibodies revealed two main classes of antibodies involved in the immune response of dogs vaccinated. While IgM antibodies were the first to appear, the succeeding IgG response was mainly IgG2 dominated independent of the vaccine type used. The results support the view that SPBN GASGAS induces a sustained detectable immune response in local dogs both after direct oral administration and via bait application.
登录
查看更多内容
影响因子：
3.8
作者：
Hampson K;Coudeville L;Lembo T;Sambo M;Kieffer A;Attlan M;Barrat J;Blanton JD;Briggs DJ;Cleaveland S;Costa P;Freuling CM;Hiby E;Knopf L;Leanes F;Meslin FX;Metlin A;Miranda ME;Müller T;Nel LH;Recuenco S;Rupprecht CE;Schumacher C;Taylor L;Vigilato MA;Zinsstag J;Dushoff J;Global Alliance for Rabies Control Partners for Rabies Prevention
通讯作者：
Global Alliance for Rabies Control Partners for Rabies Prevention
影响因子：
4.7
作者：
Freuling, Conrad M.;Kamp, Verena To;Mueller, Thomas
通讯作者：
Mueller, Thomas
影响因子：
4.6
作者：
Head, Jennifer R.;Vos, Ad;Wallace, Ryan
通讯作者：
Wallace, Ryan
影响因子：
5.5
作者：
Cliquet, F.;Gurbuxani, J. P.;Barrat, J.
通讯作者：
Barrat, J.
影响因子：
2.9
作者：
Moore SM;Gilbert A;Vos A;Freuling CM;Ellis C;Kliemt J;Müller T
通讯作者：
Müller T