Evaluation of a receptor gene responsible for maternal blood IgY transfer into egg yolks using bursectomized IgY-depleted chickens.
Evaluation of a receptor gene responsible for maternal blood IgY transfer into egg yolks using bursectomized IgY-depleted chickens.
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DOI：
10.1016/j.psj.2019.11.045
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发表时间：
2020-04
期刊：
影响因子：
4.4
通讯作者：
Horio F
中科院分区：
文献类型：
--
作者：
Murai A;Hamano T;Kakiuchi M;Kobayashi M;Horio F
在禽类中，母体免疫球蛋白Y(IgY)从血液转移到成熟卵母细胞的卵黄中；然而，这种转移的机制尚不清楚。为了深入了解母体卵黄中卵黄抗体转移的机制，在孵化过程中通过去除法氏囊(法氏囊)来产生卵黄抗体缺失的鸡，并测定了它们的产蛋量和卵黄中卵黄抗体的转运能力。孵化后，法氏囊切开雏鸡的血中IgY浓度逐渐下降，直至性成熟，而IgA浓度从生长早期(至少2周龄开始)一直保持在较低水平。通过对血液中的IgY和IgA浓度进行筛选，确定为IgY耗竭的鸡，将其提高到性成熟。在20周龄时，去卵黄组的血液和蛋黄中的IgY浓度都比对照组低600倍，而产蛋量在两组之间没有差异。静脉注射地高辛标记的卵黄中的IgY，在卵黄中的摄取率大约是对照组的2倍，这表明在成熟的卵母细胞中，卵黄中的IgY摄取率可能会增加。对包括卵母细胞核在内的生发盘的DNA微阵列分析显示，在IgY缺失组中，73个基因的表达水平上调了1.5倍以上，尽管我们无法确定一个令人信服的IgY受体候选基因。总而言之，我们成功地饲养了卵黄抗体耗竭的鸡，蛋黄抗体显著减少。注射的卵黄对卵黄的摄取增强，支持在禽类成熟的卵母细胞和卵泡中存在选择性的卵黄转运机制。
In avian species, maternal immunoglobulin Y (IgY) is transferred from the blood to the yolks of maturing oocytes; however, the mechanism underlying this transfer is unknown. To gain insight into the mechanisms of maternal IgY transfer into egg yolks, IgY-depleted chickens were generated by removing the bursa of Fabricius (bursectomy) during egg incubation, and their egg production and IgY transport ability into egg yolks were determined. After hatching, blood IgY concentrations of the bursectomized chickens decreased gradually until sexual maturity, whereas those of IgA remained low from an early stage of growth (from at least 2 wk of age). Chickens identified as depleted in IgY through screening of blood IgY and IgA concentrations were raised to sexual maturity. At 20 wk of age, both blood and egg yolk IgY concentrations in the IgY-depleted group were 600-fold lower than those of the control group, whereas egg production did not differ between the groups. Intravenously injected, digoxigenin-labeled IgY uptake into the egg yolk was approximately 2-fold higher in the IgY-depleted chickens than in the controls, suggesting that IgY depletion may enhance IgY uptake in maturing oocytes. DNA microarray analysis of the germinal disc, including the oocyte nucleus, revealed that the expression levels of 73 genes were upregulated more than 1.5-fold in the IgY-depleted group, although we could not identify a convincing candidate gene for the IgY receptor. In conclusion, we successfully raised IgY-depleted chickens presenting a marked reduction in egg yolk IgY. The enhanced uptake of injected IgY into the egg yolks of the IgY-depleted chickens supports the existence of a selective IgY transport mechanism in maturing oocytes and ovarian follicles in avian species.
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