Search for the genes involved in oocyte maturation and early embryo development in the hen.
Search for the genes involved in oocyte maturation and early embryo development in the hen.
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DOI：
10.1186/1471-2164-9-110
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发表时间：
2008-02-29
期刊：
影响因子：
4.4
通讯作者：
Govoroun MS
中科院分区：
文献类型：
--
作者：
Elis S;Batellier F;Couty I;Balzergue S;Martin-Magniette ML;Monget P;Blesbois E;Govoroun MS
发育的初始阶段取决于卵母细胞中积累的 mRNA 和蛋白质，在这些阶段，某些基因对于受精、首次卵裂和胚胎基因组激活至关重要。本研究的目的首先是使用计算机和微阵列方法寻找鸟类卵母细胞特异性基因，然后研究其中一些基因在卵泡成熟和早期胚胎发生过程中表达的时间和空间动态。计算机方法使我们能够识别通过数字差异显示发现的小鼠潜在卵母细胞基因的 18 个鸡同源物。使用鸡 Affymetrix 微阵列，我们鉴定了 461 个在母鸡卵母细胞颗粒细胞 (GC) 中过表达的基因和 250 个在母鸡卵母细胞生发盘 (GD) 中过表达的基因。使用计算机和微阵列方法鉴定了六个基因。基于GO注释，GC和GD基因在生物过程中的参与存在差异，反映了这两个细胞层不同的生理目的地。最后我们研究了21个鸡基因表达的时空动态。根据它们的表达模式，所有这些基因都参与鸡的最终卵泡成熟和/或早期胚胎发生的不同阶段。其中，8个基因（btg4、chkmos、wee、zpA、dazL、cvh、zar1和ktfn）在成熟的occyte中优先表达，cvh、zar1和ktfn在早期胚胎中也高表达。我们表明，计算机和 Affymetrix 微阵列方法是相关和互补的，以便找到可能参与卵母细胞成熟和/或早期胚胎发育的新禽类基因，并允许发现新的潜在鸡成熟卵母细胞和鸡颗粒细胞标记物，用于未来的研究。此外，对其中一些基因表达的详细研究揭示了鸡中母体效应基因的有希望的候选者。最后，有关排卵后期间 rRNA 与 mRNA 不同状态的发现揭示了母鸡卵母细胞向胚胎转变的一些机制。
The initial stages of development depend on mRNA and proteins accumulated in the oocyte, and during these stages, certain genes are essential for fertilization, first cleavage and embryonic genome activation. The aim of this study was first to search for avian oocyte-specific genes using an in silico and a microarray approaches, then to investigate the temporal and spatial dynamics of the expression of some of these genes during follicular maturation and early embryogenesis. The in silico approach allowed us to identify 18 chicken homologs of mouse potential oocyte genes found by digital differential display. Using the chicken Affymetrix microarray, we identified 461 genes overexpressed in granulosa cells (GCs) and 250 genes overexpressed in the germinal disc (GD) of the hen oocyte. Six genes were identified using both in silico and microarray approaches. Based on GO annotations, GC and GD genes were differentially involved in biological processes, reflecting different physiological destinations of these two cell layers. Finally we studied the spatial and temporal dynamics of the expression of 21 chicken genes. According to their expression patterns all these genes are involved in different stages of final follicular maturation and/or early embryogenesis in the chicken. Among them, 8 genes (btg4, chkmos, wee, zpA, dazL, cvh, zar1 and ktfn) were preferentially expressed in the maturing occyte and cvh, zar1 and ktfn were also highly expressed in the early embryo. We showed that in silico and Affymetrix microarray approaches were relevant and complementary in order to find new avian genes potentially involved in oocyte maturation and/or early embryo development, and allowed the discovery of new potential chicken mature oocyte and chicken granulosa cell markers for future studies. Moreover, detailed study of the expression of some of these genes revealed promising candidates for maternal effect genes in the chicken. Finally, the finding concerning the different state of rRNA compared to that of mRNA during the postovulatory period shed light on some mechanisms through which oocyte to embryo transition occurs in the hen.
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影响因子：
2.5
作者：
Alizadeh, Z;Kageyama, S;Aoki, F
通讯作者：
Aoki, F
DOI：
10.1016/j.bbrc.2004.09.086
发表时间：
2004-11-12
影响因子：
3.1
作者：
Dadé, S;Callebaut, I;Monget, P
通讯作者：
Monget, P
影响因子：
3.5
作者：
Dadé, S;Callebaut, I;Monget, P
通讯作者：
Monget, P
影响因子：
3.4
作者：
Dean, J
通讯作者：
Dean, J
DOI：
10.1073/pnas.111145698
发表时间：
2001-06-19
影响因子：
11.1
作者：
Bashirullah, A;Cooperstock, RL;Lipshitz, HD
通讯作者：
Lipshitz, HD