Behavioral characteristics of capsaicin mediated cutaneous, myogenic, and arthrogenic orofacial nociception in rats.
Behavioral characteristics of capsaicin mediated cutaneous, myogenic, and arthrogenic orofacial nociception in rats.
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DOI：
10.1016/j.archoralbio.2018.04.013
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发表时间：
2018-08
影响因子：
3
通讯作者：
Allen KD
中科院分区：
文献类型：
--
作者：
Rohrs EL;Neubert JK;Caudle RM;Allen KD
评估大鼠辣椒素介导的皮肤、肌源性和关节源性伤害感受与口面部触觉敏感性和咬伤相关的变化。麻醉恢复后，使用辣椒素应用后的操作测试方法评估口面部触觉敏感性和咬伤。本研究用双侧辣椒素乳膏涂抹于双侧脸颊或单独异氟醚麻醉对20只雌性cd无毛大鼠进行了实验。恢复几周后，动物（n=20）接受10 μL的单侧咬肌注射，或磷酸缓冲盐水（PBS），以评估注射致敏性。几周后，将1.0%辣椒素（10 μL）与对照剂和PBS （n=13）进行比较。几周后评估辣椒素TMJ注射。11只动物分别接受10 μL单侧颞下颌关节注射（1%）辣椒素溶液或对照物。辣椒素乳膏显著改变了皮肤的咬痕活动（使刺穿时间增加248秒（p=0.0002））和触觉灵敏度（与仅异氟醚相比，可使耐受瓶距减少0.980厘米（p=0.0001））。同样，与车辆相比，辣椒素咬肌注射增加了穿刺时间（339.6秒，p=0.07），减少了耐受瓶距（1.04 cm, p=0.005）。然而，关节内辣椒素在TMJ中只改变了啃咬（增加了133秒的穿刺时间），与车辆相比，没有发现触觉敏感性的变化。辣椒素应用于皮肤和咬肌具有相似的行为效果；然而，关节内注射TMJ只影响啃咬。这些数据表明，啮齿类动物肌源性和皮肤性疼痛模型的行为变化可能与源自颞下颌关节的关节源性疼痛模型明显不同。
To assess changes in orofacial tactile sensitivity and gnawing related to capsaicin-mediated cutaneous, myogenic, and arthrogenic nociception in the rat. After recovery from anesthesia, orofacial tactile sensitivity and gnawing were assessed using operant testing methods following capsaicin application. Twenty female CD-Hairless rats were tested with bilateral capsaicin cream application to the cheek or with isoflurane anesthesia alone. Following several weeks of recovery, animals (n=20) received either 10 μL unilateral masseter injections of vehicle, or phosphate buffered saline (PBS) to assess injection sensitization. After several weeks, masseter capsaicin (1.0%) injections (10 μL) were assessed compared to vehicle and PBS (n=13). Weeks later capsaicin TMJ injections were evaluated. Animals (n=11) received either 10 μL unilateral TMJ injections of capsaicin solution (1%) or vehicle. Capsaicin cream to the skin significantly altered gnawing activity (increased puncture time by 248 seconds (p=0.0002)) and tactile sensitivity (decreased tolerated bottle distance by 0.980 cm compared to isoflurane only (p=0.0001)). Similarly, capsaicin masseter injection increased puncture time (339.6 seconds, p=0.07) and decreased tolerated bottle distance (1.04 cm, p=0.005) compared to vehicle). However, intra-articular capsaicin in the TMJ only modified gnawing (increased puncture time by 133 seconds), with no changes found in tactile sensitivity compared to vehicle. Application of capsaicin to the skin and masseter had similar behavioral effects; however, intra-articular injections to the TMJ only affected gnawing. These data indicate the behavioral changes in rodent models of myogenic and cutaneous pain may be markedly different than models of arthrogenic pain originating from the TMJ.
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