Identification of Rice Genes Associated With Enhanced Cold Tolerance by Comparative Transcriptome Analysis With Two Transgenic Rice Plants Overexpressing DaCBF4 or DaCBF7, Isolated From Antarctic Flowering Plant Deschampsia antarctica.
Identification of Rice Genes Associated With Enhanced Cold Tolerance by Comparative Transcriptome Analysis With Two Transgenic Rice Plants Overexpressing DaCBF4 or DaCBF7, Isolated From Antarctic Flowering Plant Deschampsia antarctica.
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DOI：
10.3389/fpls.2018.00601
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发表时间：
2018
影响因子：
5.6
通讯作者：
Lee H
中科院分区：
文献类型：
--
作者：
Byun MY;Cui LH;Lee J;Park H;Lee A;Kim WT;Lee H
南极洲是生物体最恶劣的环境，很少有植物物种能够生存。 Deschampsia antarctica 是唯一适应并殖民南极海洋的天然草种。为了研究该植物适应南极的分子机制，我们鉴定并表征了 D. antarctica C 重复结合因子 4 (DaCBF4)，它属于单子叶植物 CBF IV 组。寒冷和脱水胁迫显着增加了 D. antarctica 中 DaCBF4 的转录水平。为了评估 DaCBF4 在植物中的作用，我们生成了 DaCBF4 过表达的转基因水稻植物 (Ubi:DaCBF4) 并分析了其非生物胁迫反应表型。与野生型植物相比，Ubi:DaCBF4 在任何条件下都表现出增强的冷胁迫耐受性，且没有生长迟缓。由于 Ubi:DaCBF4 的寒冷特异性表型与 Ubi:DaCBF7 (Byun 等人) 相似，因此我们通过选择两个转基因水稻品系中的差异调节基因来筛选负责提高水稻耐寒性的基因。通过使用 RNA-seq 进行比较转录组分析，我们分别鉴定了正常和冷应激条件下的 9 个和 15 个基因，作为两个 D. antarctica CBF 的假定下游靶标。总体而言，我们的结果表明，南极毛草 DaCBF4 通过调节转基因水稻植物中一组胁迫响应基因的表达水平来介导转基因水稻植物的冷胁迫反应。此外，选定的下游靶基因将可用于基因工程，以增强包括水稻在内的谷类植物的耐冷性。
Few plant species can survive in Antarctica, the harshest environment for living organisms. Deschampsia antarctica is the only natural grass species to have adapted to and colonized the maritime Antarctic. To investigate the molecular mechanism of the Antarctic adaptation of this plant, we identified and characterized D. antarctica C-repeat binding factor 4 (DaCBF4), which belongs to monocot CBF group IV. The transcript level of DaCBF4 in D. antarctica was markedly increased by cold and dehydration stress. To assess the roles of DaCBF4 in plants, we generated a DaCBF4-overexpressing transgenic rice plant (Ubi:DaCBF4) and analyzed its abiotic stress response phenotype. Ubi:DaCBF4 displayed enhanced tolerance to cold stress without growth retardation under any condition compared to wild-type plants. Because the cold-specific phenotype of Ubi:DaCBF4 was similar to that of Ubi:DaCBF7 (Byun et al.,), we screened for the genes responsible for the improved cold tolerance in rice by selecting differentially regulated genes in both transgenic rice lines. By comparative transcriptome analysis using RNA-seq, we identified 9 and 15 genes under normal and cold-stress conditions, respectively, as putative downstream targets of the two D. antarctica CBFs. Overall, our results suggest that Antarctic hairgrass DaCBF4 mediates the cold-stress response of transgenic rice plants by adjusting the expression levels of a set of stress-responsive genes in transgenic rice plants. Moreover, selected downstream target genes will be useful for genetic engineering to enhance the cold tolerance of cereal plants, including rice.
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影响因子：
5.2
作者：
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影响因子：
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影响因子：
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