The length of a junction between the B and Z conformations in DNA is three base pairs or less.
The length of a junction between the B and Z conformations in DNA is three base pairs or less.
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DNA 中 B 和 Z 构象之间的连接长度为三个碱基对或更短。
DOI：
10.1021/bi00443a032
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发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
Peticolas,WL
中科院分区：
文献类型：
--
作者：
Dai,ZY;Thomas,GA;Evertsz,E;Peticolas,WL
戴忠义，Gerald A.托马斯、伊丽莎白·埃弗茨和华纳L. Peticolas* Department of Chemistry and Institute of Molecular Biology，University of俄勒冈州，Eugene，俄勒冈州97403接收于1989年2月21日;修订版Mandarin pt接收于1989年5月4日摘要：最近有人提出，DNA序列（CG）n（A）m和它们的缀合物（T）m（CG）n之间形成的双螺旋复合物，将是在高盐浓度下在水溶液中形成BZ结的候选者[Peticolas et al.等人（1988）Proc. Acad. Sci. USA 85，2579-2583]。这种连接可能发生在d（A）md（T）m段的B型螺旋和自互补（CG）n-（CG）n序列的Z型螺旋之间。本文报道了脱氧寡核苷酸d（CGCGCGCGCGCGAAAAA）和d（CGCGCGAAAAA）及其互补物的拉曼光谱。发现后者在饱和盐溶液中不能被诱导成Z型，而前者在5.5M盐溶液中形成BZ结。比较了B和Z DNA的拉曼构象标记带AT和CG碱基对的相对强度，发现在5.5 M NaCl溶液中，AT碱基对中没有一个是Z型的，而CG碱基对中有9个是Z型的。剩下的三个CG碱基对要么在连接处要么在B形式。因此，连接由三个或更少的CG碱基对形成。如果溶液中加入95 µ NiCl 2，则整个双链体变为Z型，腺嘌呤的拉曼谱带完全变为Z型。Sheardy和Winkle [（1989）Biochemistry 28，720-725]最近报道了类似的三碱基对BZ连接，他们对B和Z束使用了不同的碱基序列。DNA-蛋白质相互作用似乎由沿着DNA的大沟和小沟沿着出现的供氢和受氢基团的序列控制（Seeman等人，1976年;贝格和冯希佩尔，1987年）。当DNA处于其典型的B形式时，氢键之间的距离
Zhongyi Dai, Gerald A. Thomas, Elisabeth Evertsz, and WarnerL. Peticolas* Department of Chemistry and Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 Received February 21, 1989; Revised Manuscript Received May 4, 1989 abstract: Recently it has been suggested that double-helical complexes formed between the DNA sequences (CG)„(A) m and their conjugates,(T) m (CG)„, would be candidates for the formation of a BZ junction in aqueous solution at high salt concentrations [Peticolas et al.(1988) Proc. Natl. Acad. Sci. USA 85, 2579-2583]. The junction was predicted to occur between a B-type helix in the d (A) md (T) m section and a Z-type helix inthe self-complementary (CG)„-(CG)„sequence. In thispaper we report Raman experiments on the deoxyoligonucleotides d (CGCGCGCGCGCGAAAAA) and d (CGCGCGAAAAA) and their com-plements. It is found the latter compound cannot be induced into the Z form in saturated salt solution but that the former sequence goes into a BZ junction at 5.5 M salt. From a comparison of the relativeintensity of the Raman conformational marker bands for B and Z DNA for both the AT and CG base pairs, it is shown that in 5.5 M NaCl solution none of the AT base pairs are in the Z form, but nine of the CG base pairs are in the Z form. The remaining three CG base pairs are either in the junction or in the B form. Thus, the junction is formed from three or less CG base pairs. If the solution is made 95 µ with NiCl2, thenthe entire duplex goes into the Z form and the Raman bands of the adenine are completely changed into those of the Z form. A similar three base pair BZ junction has recently been reported by Sheardy and Winkle [(1989) Biochemistry 28, 720-725], who used different base sequences for both the B and Z tracts. This indicates that the short BZ junction length may be independent of the base sequence.DNA-protein interaction appears to be governed by the sequence of hydrogen-donating and-accepting groups that occur along the major and minor grooves of the DNA (Seeman et al., 1976; Berg & von Hippel, 1987). When DNA is in its canonical B form, the distances between the hydrogen-bond-
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