Identification of candidate genome regions controlling disease resistance in Arachis.
Identification of candidate genome regions controlling disease resistance in Arachis.
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DOI：
10.1186/1471-2229-9-112
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发表时间：
2009-08-22
影响因子：
5.3
通讯作者：
Bertioli DJ
中科院分区：
文献类型：
--
作者：
Leal-Bertioli SC;José AC;Alves-Freitas DM;Moretzsohn MC;Guimarães PM;Nielen S;Vidigal BS;Pereira RW;Pike J;Fávero AP;Parniske M;Varshney RK;Bertioli DJ
在世界范围内，病害是花生（Arachis hypogaea）产量的重要降低因素。对许多疾病的抗性来源在栽培花生基因型中是可用的，尽管在农民喜欢的品种中通常不是。野生物种通常具有更高水平的抗性，甚至明显的免疫力，尽管农艺学上不适应的野生等位基因与野生抗病基因的连锁是不可避免的。标记辅助选择具有促进栽培和野生抗性基因座与农艺学上适应的等位基因的组合的潜力。然而，在花生中，几乎完全缺乏对控制抗病性的花生基因组区域的了解。在这项工作中，我们确定了控制抗病性的候选基因组区域。为此，我们在基于微卫星标记和豆类锚标记的花生A基因组遗传图谱上定位了晚斑病候选抗病基因和QTL。这些标记类型可分别在花生属内和其他豆科植物中转移，使得该图谱能够与其他花生图谱和其他豆科作物的图谱（包括具有测序基因组的那些）进行比对。共定位了34个序列确认的候选抗病基因和5个QTL。候选基因和QTL分布在除最小连锁群外的所有连锁群上，但分布不均匀。抗晚斑病候选基因和QTL在连锁群4的上部和连锁群2的下部区域明显聚群，表明这些区域可能控制晚斑病抗性。
Worldwide, diseases are important reducers of peanut (Arachis hypogaea) yield. Sources of resistance against many diseases are available in cultivated peanut genotypes, although often not in farmer preferred varieties. Wild species generally harbor greater levels of resistance and even apparent immunity, although the linkage of agronomically un-adapted wild alleles with wild disease resistance genes is inevitable. Marker-assisted selection has the potential to facilitate the combination of both cultivated and wild resistance loci with agronomically adapted alleles. However, in peanut there is an almost complete lack of knowledge of the regions of the Arachis genome that control disease resistance. In this work we identified candidate genome regions that control disease resistance. For this we placed candidate disease resistance genes and QTLs against late leaf spot disease on the genetic map of the A-genome of Arachis, which is based on microsatellite markers and legume anchor markers. These marker types are transferable within the genus Arachis and to other legumes respectively, enabling this map to be aligned to other Arachis maps and to maps of other legume crops including those with sequenced genomes. In total, 34 sequence-confirmed candidate disease resistance genes and five QTLs were mapped. Candidate genes and QTLs were distributed on all linkage groups except for the smallest, but the distribution was not even. Groupings of candidate genes and QTLs for late leaf spot resistance were apparent on the upper region of linkage group 4 and the lower region of linkage group 2, indicating that these regions are likely to control disease resistance.
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影响因子：
4.4
作者：
Fredslund J;Madsen LH;Hougaard BK;Nielsen AM;Bertioli D;Sandal N;Stougaard J;Schauser L
通讯作者：
Schauser L
PRIFI：使用相关序列的多重比对来找到用于放大同源物的引物。
影响因子：
14.9
作者：
Fredslund, J;Schauser, L;Madsen, LH;Sandal, N;Stougaard, J
通讯作者：
Stougaard, J
Gemprospector-杂货和草丛中跨物种遗传标记的候选者的设计。
影响因子：
14.9
作者：
Fredslund, Jakob;Madsen, Lene H.;Hougaard, Birgit K.;Sandal, Niels;Stougaard, Jens;Bertioli, David;Schauser, Leif
通讯作者：
Schauser, Leif
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
3.1
作者：
Graham, MA;Marek, LF;Shoemaker, RC
通讯作者：
Shoemaker, RC