A novel photic entrainment mechanism for the circadian clock in an insect: involvement of c-fos and cryptochromes.
A novel photic entrainment mechanism for the circadian clock in an insect: involvement of c-fos and cryptochromes.
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DOI：
10.1186/s40851-018-0109-8
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发表时间：
2018
影响因子：
2.7
通讯作者：
Tomioka K
中科院分区：
文献类型：
--
作者：
Kutaragi Y;Tokuoka A;Tomiyama Y;Nose M;Watanabe T;Bando T;Moriyama Y;Tomioka K
对环境光周期的干扰是生物钟的基本属性。虽然已知复眼是许多昆虫的主要光感受器，但光夹带的分子机制仍有待探索。我们发现隐色素（crys）和c-fos介导半代谢昆虫蟋蟀Gryllus bimaculatus生物钟的光干扰。我们研究了RNA干扰（RNAi）介导的cry基因Gb 'cry1和Gb 'cry2的敲低对光诱导和光诱导的昼夜运动节律重置的影响。Gb 'cry2 RNAi加速了延迟转移的夹带，而Gb 'cry1 / Gb 'cry2双RNAi在某些蟋蟀的提前和延迟转移中都导致瞬态周期的显著延长，甚至导致夹带失败。双RNAi也强烈抑制光诱导复位。在光诱导的Gb 'c-fosB表达之前，Gb ' cry介导的相移或节律重置。我们还发现Gb 'c-fosB, Gb 'cry2和Gb 'period （Gb 'per）可能在一些视叶神经元中共表达。基于这些结果，我们提出了一种新的昆虫生物钟光干扰模型，该模型依赖于复眼感知的光信息。本文的在线版本（10.1186/s40851-018-0109-8）包含补充材料，可供授权用户使用。
Entrainment to the environmental light cycle is an essential property of the circadian clock. Although the compound eye is known to be the major photoreceptor necessary for entrainment in many insects, the molecular mechanisms of photic entrainment remain to be explored. We found that cryptochromes (crys) and c-fos mediate photic entrainment of the circadian clock in a hemimetabolous insect, the cricket Gryllus bimaculatus. We examined the effects of RNA interference (RNAi)-mediated knockdown of the cry genes, Gb’cry1 and Gb’cry2, on photic entrainment, and light-induced resetting of the circadian locomotor rhythm. Gb’cry2 RNAi accelerated entrainment for delay shifts, while Gb’cry1/ Gb’cry2 double RNAi resulted in significant lengthening of transient cycles in both advance and delay shifts, and even in entrainment failure in some crickets. Double RNAi also strongly suppressed light induced resetting. The Gb’cry-mediated phase shift or resetting of the rhythm was preceded by light-induced Gb’c-fosB expression. We also found that Gb’c-fosB, Gb’cry2 and Gb’period (Gb’per) were likely co-expressed in some optic lobe neurons. Based on these results, we propose a novel model for photic entrainment of the insect circadian clock, which relies on the light information perceived by the compound eye. The online version of this article (10.1186/s40851-018-0109-8) contains supplementary material, which is available to authorized users.
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影响因子：
3.7
作者：
Mracek P;Santoriello C;Idda ML;Pagano C;Ben-Moshe Z;Gothilf Y;Vallone D;Foulkes NS
通讯作者：
Foulkes NS
原始昆虫中的昼夜节律需要核受体基因HR3和E75。
影响因子：
3.7
作者：
Kamae Y;Uryu O;Miki T;Tomioka K
通讯作者：
Tomioka K
影响因子：
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通讯作者：
Partch CL
影响因子：
16.2
作者：
KORNHAUSER, JM;NELSON, DE;TAKAHASHI, JS
通讯作者：
TAKAHASHI, JS
影响因子：
3.5
作者：
Miyasako, Yoko;Umezaki, Yujiro;Tomioka, Kenji
通讯作者：
Tomioka, Kenji