A practical guide to rodent islet isolation and assessment.
A practical guide to rodent islet isolation and assessment.
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DOI：
10.1007/s12575-009-9021-0
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发表时间：
2009-12-03
影响因子：
6.4
通讯作者：
Nunemaker CS
中科院分区：
文献类型：
--
作者：
Carter JD;Dula SB;Corbin KL;Wu R;Nunemaker CS
朗格汉斯胰岛分泌对血糖调节至关重要的激素，因此是糖尿病研究的重点。从胰腺中纯化有活力和功能的胰岛用于研究是一个复杂的过程。本文综述了小鼠和大鼠胰岛分离的关键因素，包括胶原酶的选择，胶原酶消化过程，利用密度梯度纯化胰岛，以及胰岛培养条件。此外，本文综述了评估胰岛活力和功能的常用技术，包括视觉评估、细胞死亡荧光标记、葡萄糖刺激胰岛素分泌和细胞内钙测量。还包括详细的协议，描述了一种常见的啮齿动物胰岛分离方法，我们的实验室使用该方法获得可行的和功能性的小鼠胰岛，用于体外研究胰岛功能、β细胞生理学和体内啮齿动物胰岛移植。本综述的目的是为成功获取和纯化高质量胰岛提供资源和基础。
Pancreatic islets of Langerhans secrete hormones that are vital to the regulation of blood glucose and are, therefore, a key focus of diabetes research. Purifying viable and functional islets from the pancreas for study is an intricate process. This review highlights the key elements involved with mouse and rat islet isolation, including choices of collagenase, the collagenase digestion process, purification of islets using a density gradient, and islet culture conditions. In addition, this paper reviews commonly used techniques for assessing islet viability and function, including visual assessment, fluorescent markers of cell death, glucose-stimulated insulin secretion, and intracellular calcium measurements. A detailed protocol is also included that describes a common method for rodent islet isolation that our laboratory uses to obtain viable and functional mouse islets for in vitro study of islet function, beta-cell physiology, and in vivo rodent islet transplantation. The purpose of this review is to serve as a resource and foundation for successfully procuring and purifying high-quality islets for research purposes.
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影响因子：
4.8
作者：
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