Antagonistic Effect of Sucrose Availability and Auxin on Rosa Axillary Bud Metabolism and Signaling, Based on the Transcriptomics and Metabolomics Analysis.
Antagonistic Effect of Sucrose Availability and Auxin on Rosa Axillary Bud Metabolism and Signaling, Based on the Transcriptomics and Metabolomics Analysis.
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DOI：
10.3389/fpls.2022.830840
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发表时间：
2022
影响因子：
5.6
通讯作者：
Sakr S
中科院分区：
文献类型：
--
作者：
Wang M;Ogé L;Pérez Garcia MD;Launay-Avon A;Clément G;Le Gourrierec J;Hamama L;Sakr S
茎部分枝是植物发育成功和对环境因子响应的关键。广泛的调查揭示了包括激素和糖在内的一个复杂的调节网络的参与。近年来的研究表明，生长素和糖这两种主要的系统调节剂对植物的分枝有拮抗作用。然而，人们对这种串扰的分子机制知之甚少。我们对外植体茎芽进行了两种互补的非靶向方法——rna -seq和代谢组学——分别饲喂不同浓度的生长素和蔗糖，得到休眠和非休眠芽。芽通过转录组和代谢组的大量重编程来响应生长素和糖的联合作用。蔗糖和生长素的拮抗作用针对几个重要的生理过程，包括汇强度、氨基酸代谢、硫酸盐代谢、核糖体生物生成、核酸代谢和植物激素信号传导。进一步的实验揭示了tor激酶信号通路至少通过下调Rosa hybrida BRANCHED1 （RhBRC1）在芽生长中的作用。这些新发现为进一步研究在茎枝分枝过程中驱动内源因子整合的多种分子机制奠定了基础。
Shoot branching is crucial for successful plant development and plant response to environmental factors. Extensive investigations have revealed the involvement of an intricate regulatory network including hormones and sugars. Recent studies have demonstrated that two major systemic regulators—auxin and sugar—antagonistically regulate plant branching. However, little is known regarding the molecular mechanisms involved in this crosstalk. We carried out two complementary untargeted approaches—RNA-seq and metabolomics—on explant stem buds fed with different concentrations of auxin and sucrose resulting in dormant and non-dormant buds. Buds responded to the combined effect of auxin and sugar by massive reprogramming of the transcriptome and metabolome. The antagonistic effect of sucrose and auxin targeted several important physiological processes, including sink strength, the amino acid metabolism, the sulfate metabolism, ribosome biogenesis, the nucleic acid metabolism, and phytohormone signaling. Further experiments revealed a role of the TOR-kinase signaling pathway in bud outgrowth through at least downregulation of Rosa hybrida BRANCHED1 (RhBRC1). These new findings represent a cornerstone to further investigate the diverse molecular mechanisms that drive the integration of endogenous factors during shoot branching.
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影响因子：
5.6
作者：
Aklilu BB;Culligan KM
通讯作者：
Culligan KM
影响因子：
6.9
作者：
Clément G;Moison M;Soulay F;Reisdorf-Cren M;Masclaux-Daubresse C
通讯作者：
Masclaux-Daubresse C
影响因子：
13.8
作者：
Burkart GM;Brandizzi F
通讯作者：
Brandizzi F
影响因子：
2.2
作者：
Chua, Siang Ling;Too, Wei Cun See;Few, Ling Ling
通讯作者：
Few, Ling Ling
影响因子：
11.6
作者：
Aguilar-Martinez, Jose Antonio;Poza-Carrion, Cesar;Cubas, Pilar
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Cubas, Pilar