The potential and challenges of nanopore sequencing.
The potential and challenges of nanopore sequencing.
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DOI：
10.1038/nbt.1495
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发表时间：
2008-10
影响因子：
46.9
通讯作者：
Schloss, Jeffery A.
中科院分区：
文献类型：
--
作者：
Branton, Daniel;Deamer, David W.;Marziali, Andre;Bayley, Hagan;Benner, Steven A.;Butler, Thomas;Di Ventra, Massimiliano;Garaj, Slaven;Hibbs, Andrew;Huang, Xiaohua;Jovanovich, Stevan B.;Krstic, Predrag S.;Lindsay, Stuart;Ling, Xinsheng Sean;Mastrangelo, Carlos H.;Meller, Amit;Oliver, John S.;Pershin, Yuriy V.;Ramsey, J. Michael;Riehn, Robert;Soni, Gautam V.;Tabard-Cossa, Vincent;Wanunu, Meni;Wiggin, Matthew;Schloss, Jeffery A.
基于纳米孔的装置提供单分子检测和分析能力，其通过将溶液中的分子通过纳米级孔进行电驱动来实现。纳米孔提供了高度受限的空间，在该空间内可以通过多种手段之一以高通量分析单个核酸聚合物，并且可以在窄孔中实施的完美的持续合成能力确保了多核苷酸中核碱基的天然顺序反映在检测到的信号序列中。千碱基长度聚合物（单链基因组DNA或RNA）或小分子（例如，核苷）可以在没有扩增或标记的情况下被识别和表征，这是一种独特的分析能力，使得廉价、快速的DNA测序成为可能。进一步研究和开发，以克服目前的挑战，纳米孔鉴定DNA链中的每个连续的核苷酸提供了前景的“第三代”仪器，将测序二倍体哺乳动物基因组的约1,000美元在24小时。
A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore. The nanopore provides a highly confined space within which single nucleic acid polymers can be analyzed at high throughput by one of a variety of means, and the perfect processivity that can be enforced in a narrow pore ensures that the native order of the nucleobases in a polynucleotide is reflected in the sequence of signals that is detected. Kilobase length polymers (single-stranded genomic DNA or RNA) or small molecules (e.g., nucleosides) can be identified and characterized without amplification or labeling, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility. Further research and development to overcome current challenges to nanopore identification of each successive nucleotide in a DNA strand offers the prospect of `third generation' instruments that will sequence a diploid mammalian genome for ~$1,000 in ~24 h.
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影响因子：
15
作者：
Cockroft, Scott L.;Chu, John;Ghadiri, M. Reza
通讯作者：
Ghadiri, M. Reza
影响因子：
10.8
作者：
Fologea, D;Uplinger, J;Li, JL
通讯作者：
Li, JL
影响因子：
10.8
作者：
Fologea, D;Gershow, M;Li, JL
通讯作者：
Li, JL
DOI：
10.1016/j.physa.2006.08.068
发表时间：
2007-01-01
影响因子：
3.3
作者：
Cheikh, Christophe;Koper, Ger
通讯作者：
Koper, Ger
影响因子：
13.3
作者：
Chen, Peng;Li, Chang Ming
通讯作者：
Li, Chang Ming