A Unique Tryptophan C-Prenyltransferase from the Kawaguchipeptin Biosynthetic Pathway.
A Unique Tryptophan C-Prenyltransferase from the Kawaguchipeptin Biosynthetic Pathway.
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来自川单肽生物合成途径的独特的色氨酸C-丙基转移酶。
DOI：
10.1002/anie.201509920
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发表时间：
2016-03-07
影响因子：
16.6
通讯作者：
Fewer, David Peter
中科院分区：
文献类型：
--
作者：
Parajuli, Anirudra;Kwak, Daniel H.;Dalponte, Luca;Leikoski, Niina;Galica, Tomas;Umeobika, Ugochukwu;Trembleau, Laurent;Bent, Andrew;Sivonen, Kaarina;Wahlsten, Matti;Wang, Hao;Rizzi, Ermanno;De Bellis, Gianluca;Naismith, James;Jaspars, Marcel;Liu, Xinyu;Houssen, Wael;Fewer, David Peter
蓝细菌素是由蓝细菌产生的一类快速增长的线性和环状肽。河口肽A和B是铜绿微囊藻NIES-88中的两种大环十一肽，它们是氰氧化菌素生物合成途径的产物。在铜绿微囊藻（Microcystis aeruginosa）NIES-88的5.26 Mb草图基因组中鉴定出9 kb的河口抑肽酶（kawaguchipeptin，kgp）基因簇。我们证实，该基因簇是负责通过在大肠杆菌中异源表达的kgp基因簇的kawaguchipeptin的生产。KgpF异戊烯基转移酶过表达，并显示在体外线性和环状肽中的色氨酸残基的C-3异戊烯基化。我们的研究结果有助于进一步提高蓝藻的结构多样性，包括异戊烯化的环肽。
Cyanobactins are are rapidly growing family of linear and cyclic peptides produced by cyanobacteria. Kawaguchipeptins A and B, two macrocyclic undecapeptides reported earlier from Microcystis aeruginosa NIES-88, are shown to be products of the cyanobactin biosynthetic pathway. The 9 kb kawaguchipeptin (kgp) gene cluster was identified in a 5.26 Mb draft genome of Microcystis aeruginosa NIES-88. We verified that this gene cluster is responsible for the production of the kawaguchipeptins through heterologous expression of the kgp gene cluster in Escherichia coli. The KgpF prenyltransferase was overexpressed and was shown to prenylate C-3 of Trp residues in both linear and cyclic peptides in vitro. Our findings serve to further enhance the structural diversity of cyanobactins to include tryptophan-prenylated cyclic peptides.
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影响因子：
16.6
作者：
Houssen, Wael E.;Bent, Andrew F.;McEwan, Andrew R.;Pieiller, Nathalie;Tabudravu, Jioji;Koehnke, Jesko;Mann, Greg;Adaba, Rosemary I.;Thomas, Louise;Hawas, Usama W.;Liu, Huanting;Schwarz-Linek, Ulrich;Smith, Margaret C. M.;Naismith, James H.;Jaspars, Marcel
通讯作者：
Jaspars, Marcel
DOI：
10.1107/s1744309113012931
发表时间：
2013-06
期刊：
Acta crystallographica. Section F, Structural biology and crystallization communications
影响因子：
--
作者：
Bent AF;Koehnke J;Houssen WE;Smith MC;Jaspars M;Naismith JH
通讯作者：
Naismith JH
影响因子：
4.4
作者：
Leikoski, Niina;Fewer, David P.;Sivonen, Kaarina
通讯作者：
Sivonen, Kaarina
影响因子：
14.8
作者：
Donia, Mohamed S.;Hathaway, Brian J.;Schmidt, Eric W.
通讯作者：
Schmidt, Eric W.
影响因子：
14.8
作者：
Donia, Mohamed S.;Ravel, Jacques;Schmidt, Eric W.
通讯作者：
Schmidt, Eric W.