Genetic interactions between ABA signalling and the Arg/N-end rule pathway during Arabidopsis seedling establishment.
Genetic interactions between ABA signalling and the Arg/N-end rule pathway during Arabidopsis seedling establishment.
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DOI：
10.1038/s41598-018-33630-5
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发表时间：
2018-10-12
影响因子：
4.6
通讯作者：
Theodoulou FL
中科院分区：
文献类型：
--
作者：
Zhang H;Gannon L;Jones PD;Rundle CA;Hassall KL;Gibbs DJ;Holdsworth MJ;Theodoulou FL
泛素介导的蛋白质降解的Arg/N末端调节通路在植物发育过程中具有多种功能，尤其是在休眠种子向光自养幼苗的转变过程中。ProteolySIS6(PRT6)是Arg/N-End规则中的N-Recognin E3连接酶，调节属于乙烯反应因子超家族(ERFVIIs)第VII组的转录因子底物的降解。目前尚不清楚ERFVII是否与Arg/N-end规则的所有已知功能相关，也不完全定义受ERFVII影响的下游通路。在此，我们研究了PRT6功能、ERFVII和ABA信号在拟南芥幼苗建立中的关系。对幼苗的生理分析表明，5个ERFVII中的3个，RAP2.12，RAP2.2和RAP2.3中的N端规则调控的稳定化，控制了幼苗形成和萌发后油体分解的糖敏感性。在含有稳定的ERFVII的背景下，ABA信号成分ABA不敏感(ABI)4以及ABI3和ABI5被发现提高了ABA对萌发的敏感性和对建立的糖的敏感性。然而，N-端规则对油体的调节并不依赖于典型的ABA信号。我们认为，N-端规则通过调节ERFVII活性来控制种子到幼苗转换的多个方面，涉及ABA依赖和独立的信号通路。
The Arg/N-end rule pathway of ubiquitin-mediated proteolysis has multiple functions throughout plant development, notably in the transition from dormant seed to photoautotrophic seedling. PROTEOLYSIS6 (PRT6), an N-recognin E3 ligase of the Arg/N-end rule regulates the degradation of transcription factor substrates belonging to Group VII of the Ethylene Response Factor superfamily (ERFVIIs). It is not known whether ERFVIIs are associated with all known functions of the Arg/N-end rule, and the downstream pathways influenced by ERFVIIs are not fully defined. Here, we examined the relationship between PRT6 function, ERFVIIs and ABA signalling in Arabidopsis seedling establishment. Physiological analysis of seedlings revealed that N-end rule-regulated stabilisation of three of the five ERFVIIs, RAP2.12, RAP2.2 and RAP2.3, controls sugar sensitivity of seedling establishment and oil body breakdown following germination. ABA signalling components ABA INSENSITIVE (ABI)4 as well as ABI3 and ABI5 were found to enhance ABA sensitivity of germination and sugar sensitivity of establishment in a background containing stabilised ERFVIIs. However, N-end rule regulation of oil bodies was not dependent on canonical ABA signalling. We propose that the N-end rule serves to control multiple aspects of the seed to seedling transition by regulation of ERFVII activity, involving both ABA-dependent and independent signalling pathways.
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影响因子：
4.6
作者：
de Marchi R;Sorel M;Mooney B;Fudal I;Goslin K;Kwaśniewska K;Ryan PT;Pfalz M;Kroymann J;Pollmann S;Feechan A;Wellmer F;Rivas S;Graciet E
通讯作者：
Graciet E
影响因子：
5.1
作者：
Dekkers, Bas J. W.;Schuurmans, Jolanda A. M. J.;Smeekens, Sjef C. M.
通讯作者：
Smeekens, Sjef C. M.
影响因子：
7.4
作者：
Baud, Sebastien;Kelemen, Zsolt;Dubreucq, Bertrand
通讯作者：
Dubreucq, Bertrand
影响因子：
7.4
作者：
Brocard, IM;Lynch, TJ;Finkelstein, RR
通讯作者：
Finkelstein, RR
影响因子：
11.6
作者：
GIRAUDAT, J;HAUGE, BM;GOODMAN, HM
通讯作者：
GOODMAN, HM