Three-dimensional transmural organization of perimysial collagen in the heart.
Three-dimensional transmural organization of perimysial collagen in the heart.
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DOI：
10.1152/ajpheart.00484.2008
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发表时间：
2008-09
期刊：
影响因子：
--
通讯作者：
LeGrice IJ
中科院分区：
文献类型：
--
作者：
Pope AJ;Sands GB;Smaill BH;LeGrice IJ
有强有力的支持，认为心室肌有一个层状组织，其中肌细胞被分组为分裂面分开的分支层。然而，这种结构的程度和功能的影响的理解一直受到限制，缺乏一个系统的三维描述的组织的肌细胞和相关的肌周胶原。我们成像心肌细胞和胶原蛋白在高分辨率在7个正常大鼠心脏左心室壁使用扩展体积共聚焦显微镜。我们开发了新的重建和分割技术所需的三维肌细胞和肌周胶原组织的定量分析。结果证实，肌束膜胶原蛋白具有有序的排列，它定义了一个层状组织。肌周胶原蛋白由三种不同的形式组成：层状表面上的广泛网状结构、连接相邻层的卷曲纤维和纵向索。虽然肌层是大部分壁的主要结构组织形式，但在心外膜下看不到肌层，肌周胶原仅以纵索形式存在。
There is strong support for the view that the ventricular myocardium has a laminar organization in which myocytes are grouped into branching layers separated by cleavage planes. However, understanding of the extent and functional implications of this architecture has been limited by the lack of a systematic three-dimensional description of the organization of myocytes and associated perimysial collagen. We imaged myocytes and collagen across the left ventricular wall at high resolution in seven normal rat hearts using extended volume confocal microscopy. We developed novel reconstruction and segmentation techniques necessary for the quantitative analysis of three-dimensional myocyte and perimysial collagen organization. The results confirm that perimysial collagen has an ordered arrangement and that it defines a laminar organization. Perimysial collagen is composed of three distinct forms: extensive meshwork on laminar surfaces, convoluted fibers connecting adjacent layers, and longitudinal cords. While myolaminae are the principal form of structural organization throughout most of the wall, they are not seen in the subepicardium, where perimysial collagen is present only as longitudinal cords.
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DOI：
10.1152/ajpheart.00355.2006
发表时间：
2007-03-01
影响因子：
4.8
作者：
Graham, H. K.;Trafford, A. W.
通讯作者：
Trafford, A. W.
影响因子：
9.5
作者：
MacKenna, DA;Omens, JH;Covell, JW
通讯作者：
Covell, JW
影响因子：
3.4
作者：
Kocica, Mladen J.;Corno, Antonio F.;Torrent-Guasp, Francisco
通讯作者：
Torrent-Guasp, Francisco
影响因子：
3.3
作者：
Beg, MF;Helm, PA;Winslow, RL
通讯作者：
Winslow, RL
DOI：
10.1196/annals.1341.026
发表时间：
2005-01-01
期刊：
COMMUNICATIVE CARDIAC CELL
影响因子：
--
作者：
Helm, P;Beg, MF;Winslow, RL
通讯作者：
Winslow, RL