Seed-mediated gene flow promotes genetic diversity of weedy rice within populations: implications for weed management.
Seed-mediated gene flow promotes genetic diversity of weedy rice within populations: implications for weed management.
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DOI：
10.1371/journal.pone.0112778
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Lu BR
中科院分区：
文献类型：
--
作者：
He Z;Jiang X;Ratnasekera D;Grassi F;Perera U;Lu BR
杂草稻是一种有害的农业害虫，其危害日益严重，已造成世界范围内栽培稻产量的显著下降。杂草稻种群的遗传多样性和结构的知识将有助于设计有效的方法来控制这种杂草，通过跟踪其起源和传播模式在一个给定的区域。为了产生这样的知识，我们研究了遗传多样性和结构的21杂草稻种群从斯里兰卡的基础上选择的23个微卫星（SSR）位点。结果表明，一个非常高的水平的群体内的遗传多样性（He = 0.62）和有限的种群分化（Fst = 0.17），这主要是自花授粉杂草。 UPGMA分析表明，全国杂草稻居群间的遗传距离与地理位置的关系较为松散，居群间的遗传结构不明显。这种现象与种群间大量的基因流动有关。有限的混合物结构分析表明，一个非常低的杂交水平（花粉介导的基因流）之间的人口。丰富的群体内遗传多样性加上有限的群体遗传结构和分化可能是由于相当大的种子介导的基因流杂草稻沿着在斯里兰卡杂草稻污染地区之间的远距离交换农民保存的水稻种子。除了其他有效的杂草管理策略外，推广应用无杂草稻污染的认证稻种应是立即采取的行动，以显著减少这种杂草在斯里兰卡等水稻种植方式相似的国家的水稻生态系统中的扩散和侵扰。
Increased infestation of weedy rice—a noxious agricultural pest has caused significant reduction of grain yield of cultivated rice (Oryza sativa) worldwide. Knowledge on genetic diversity and structure of weedy rice populations will facilitate the design of effective methods to control this weed by tracing its origins and dispersal patterns in a given region. To generate such knowledge, we studied genetic diversity and structure of 21 weedy rice populations from Sri Lanka based on 23 selected microsatellite (SSR) loci. Results indicated an exceptionally high level of within-population genetic diversity (He = 0.62) and limited among-population differentiation (Fst = 0.17) for this predominantly self-pollinating weed. UPGMA analysis showed a loose genetic affinity of the weedy rice populations in relation to their geographical locations, and no obvious genetic structure among populations across the country. This phenomenon was associated with the considerable amount of gene flow between populations. Limited admixture from STRUCTURE analyses suggested a very low level of hybridization (pollen-mediated gene flow) between populations. The abundant within-population genetic diversity coupled with limited population genetic structure and differentiation is likely caused by the considerable seed-mediated gene flow of weedy rice along with the long-distance exchange of farmer-saved rice seeds between weedy-rice contaminated regions in Sri Lanka. In addition to other effective weed management strategies, promoting the application of certified rice seeds with no weedy rice contamination should be the immediate action to significantly reduce the proliferation and infestation of this weed in rice ecosystems in countries with similar rice farming styles as in Sri Lanka.
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