Sodium influx induced by external calcium chelation decreases human sperm motility.
Sodium influx induced by external calcium chelation decreases human sperm motility.
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外部钙螯合引起的钠流入会降低人类精子的活力。
DOI：
10.1093/humrep/der237
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Gonzalez-Martinez,MarcoT
中科院分区：
文献类型：
--
作者：
Torres-Flores,Victor;Picazo-Juarez,Giovanni;Hernandez-Rueda,Yadira;Darszon,Alberto;Gonzalez-Martinez,MarcoT
背景从培养基中去除钙会迅速降低人类精子活力并诱导Na+依赖性去极化，并伴有细胞内钠浓度（[Na+]i）增加和细胞内钙浓度（[Ca 2 +]i）降低。方法用荧光探针diSC 3（5）和fura 2同时检测人精子膜电位（Vm）和[Ca 2 +] i。[Na+] i和以类似的方式使用钠结合苯并呋喃三乙酸盐独立地测量。在CASA系统中测定运动性，使用荧光素酶测定法测量ATP，并通过放射免疫测定法测量cAMP。在钙去除后，人精子运动性降低与Na+-负荷或Na+-依赖性去极化有关，因为在抑制钙去除诱导的Na+-依赖性去极化和[Na+] i增加的条件下，精子运动性不受影响。通过用缬氨霉素夹持精子Vm，我们发现与钙去除相关的运动性降低与钠负荷有关，而不是与膜电位去极化有关。钙通道阻滞剂米贝拉地尔可明显抑制Na+依赖性去极化和钠负荷，并能保护精子活力。在没有钙的情况下，ATP和cAMP浓度均降低40%。然而，ATP水平不变时，钙去除条件下进行抑制钙去除诱导的Na+依赖性去极化和[Na+] i increasing. CONCLUSIONSHAN人精子运动阻滞引起的外部钙去除介导的主要是由钠负荷，这将刺激Na+/K+-ATP酶，从而耗尽ATP含量。
BACKGROUNDCalcium removal from the medium promptly reduces human sperm motility and induces a Na+-dependent depolarization that is accompanied by an increase in intracellular sodium concentration ([Na+]i) and a decrease in intracellular calcium concentration ([Ca2+]i). Sodium loading activates a Na+/K+-ATPase.METHODSMembrane potential (Vm) and [Ca2+]iwere simultaneously detected in human sperm populations with the fluorescent probes diSC3(5) and fura 2. [Na+]iand was measured independently in a similar fashion using sodium-binding benzofuran isophthalate. Motility was determined in a CASA system, ATP was measured using the luciferin-luciferase assay, and cAMP was measured by radioimmunoassay.RESULTSHuman sperm motility reduction after calcium removal is related to either Na+-loading or Na+-dependent depolarization, because, under conditions that inhibit the calcium removal-induced Na+-dependent depolarization and [Na+]iincrease, sperm motility was unaffected. By clamping sperm Vm with valinomycin, we found that the motility reduction associated with the calcium removal was related to sodium loading, and not to membrane potential depolarization. Mibefradil, a calcium channel blocker, markedly inhibited the Na+-dependent depolarization and sodium loading, and also preserved sperm motility. In the absence of calcium, both ATP and cAMP concentrations were decreased by 40%. However ATP levels were unchanged when calcium removal was performed under conditions that inhibit the calcium removal-induced Na+-dependent depolarization and [Na+]iincrease.CONCLUSIONSHuman sperm motility arrest induced by external calcium removal is mediated principally by sodium loading, which would stimulate the Na+/K+-ATPase and in turn deplete the ATP content.
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DOI：
--
发表时间：
1986
期刊：
影响因子：
--
作者：
S. Suarez;D. Wolf;S. Meizel
通讯作者：
S. Meizel
影响因子：
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作者：
J. Gomora;J. A. Enyeart;J. J. Enyeart-J.
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J. Gomora;J. A. Enyeart;J. J. Enyeart-J.
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作者：
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Kaupp, U. Benjamin
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DOI：
--
发表时间：
1998
期刊：
Molecular pharmacology.
影响因子：
--
作者：
Arnoult,C;Villaz,M;Florman,HM
通讯作者：
Florman,HM
影响因子：
6.6
作者：
AABERG, RA;SAUER, MV;RAJFER, J
通讯作者：
RAJFER, J