Spindle checkpoint activation at meiosis I advances anaphase II onset via meiosis-specific APC/C regulation.
Spindle checkpoint activation at meiosis I advances anaphase II onset via meiosis-specific APC/C regulation.
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DOI：
10.1083/jcb.200802053
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发表时间：
2008-07-28
影响因子：
7.8
通讯作者：
Hiraoka, Yasushi
中科院分区：
文献类型：
--
作者：
Yamamoto, Ayumu;Kitamura, Kenji;Hihara, Daisuke;Hirose, Yukinobu;Katsuyama, Satoshi;Hiraoka, Yasushi
在有丝分裂过程中，纺锤体组装检查点（SAC）抑制cdc20激活的后期促进复合体/环体（APC/CCdc20），促进蛋白质降解，并延迟后期开始，以确保准确的染色体分离。然而，SAC在减数分裂后期调控中的作用尚不清楚。在这里，我们研究了SAC在分裂酵母减数分裂中的作用。与有丝分裂一样，当染色体不正确地附着在纺锤体上时，SAC因子Mad2通过Slp1（裂变酵母Cdc20）延迟了后期的开始。然而，当SAC延迟I后期时，减数分裂I和II之间的间隔缩短。此外，减数分裂期后期开始提前，SAC效应减弱。晚期发病的推进依赖于减数分裂特异性的cdc20相关因子Fzr1/Mfr1，该因子促进了晚期周期蛋白的下降和晚期发病，但SAC无法有效抑制该因子。我们的研究结果表明，由于减数分裂特异性APC/C调控，SAC激活的影响并不局限于减数分裂中的单个分裂，这可能是为了执行两次减数分裂而进化的。
During mitosis, the spindle assembly checkpoint (SAC) inhibits the Cdc20-activated anaphase-promoting complex/cyclosome (APC/CCdc20), which promotes protein degradation, and delays anaphase onset to ensure accurate chromosome segregation. However, the SAC function in meiotic anaphase regulation is poorly understood. Here, we examined the SAC function in fission yeast meiosis. As in mitosis, a SAC factor, Mad2, delayed anaphase onset via Slp1 (fission yeast Cdc20) when chromosomes attach to the spindle improperly. However, when the SAC delayed anaphase I, the interval between meiosis I and II shortened. Furthermore, anaphase onset was advanced and the SAC effect was reduced at meiosis II. The advancement of anaphase onset depended on a meiosis-specific, Cdc20-related factor, Fzr1/Mfr1, which contributed to anaphase cyclin decline and anaphase onset and was inefficiently inhibited by the SAC. Our findings show that impacts of SAC activation are not confined to a single division at meiosis due to meiosis-specific APC/C regulation, which has probably been evolved for execution of two meiotic divisions.
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DOI：
10.1083/jcb.200605074
发表时间：
2006-08-14
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ding DQ;Sakurai N;Katou Y;Itoh T;Shirahige K;Haraguchi T;Hiraoka Y
通讯作者：
Hiraoka Y
影响因子：
10.5
作者：
CohenFix, O;Peters, JM;Koshland, D
通讯作者：
Koshland, D
影响因子：
9.2
作者：
Jacobs, HW;Richter, DO;Lehner, CF
通讯作者：
Lehner, CF
影响因子：
64.8
作者：
Izawa, D;Goto, M;Yamamoto, M
通讯作者：
Yamamoto, M
影响因子：
2.1
作者：
Nakase, Y;Nakamura, T;Shimoda, C
通讯作者：
Shimoda, C